# In broodmares undergoing artificial insemination is intrauterine fluid development more likely with frozen semen or chilled semen?

**Authors:** Rumaysa Bint Saifullah+, Mary-Kate Burke+

PMC · DOI: 10.18849/ve.v10i4.728 · 2025-12-04

## TL;DR

This paper investigates whether using frozen or chilled semen in horse breeding leads to more fluid buildup in mares after artificial insemination.

## Contribution

The study compares frozen and chilled semen effects on intrauterine fluid in mares using retrospective data.

## Key findings

- Two studies found lower intrauterine fluid rates with frozen semen.
- One study found higher intrauterine fluid rates with frozen semen.
- Overall evidence is weak and inconsistent.

## Abstract

In broodmares undergoing artificial insemination, is intrauterine fluid development more likely postinsemination with frozen semen compared to insemination with chilled semen?

Incidence.

Three retrospective cohort studies were critically appraised.

Weak.

A lower rate of postbreeding intrauterine fluid occurred in mares inseminated with frozen semen compared to chilled semen in two studies, and a higher rate in one study.

The evidence available is weak and warrants further research into postbreeding intrauterine fluid rates in mares inseminated with frozen and chilled semen.

## Linked entities

- **Species:** Equus caballus (taxon 9796)

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Source: https://tomesphere.com/paper/PMC13011001