Polyethylene Mulch Emissions Differentially Impact the Soil Metabolome and Microbial Community in Field Pea (Pisum sativum L.) Cultivation

TL;DR

This study shows that polyethylene mulch affects soil chemistry and microbes, boosting bacteria but reducing fungi, which could impact soil health.

Contribution

The study reveals novel insights into how polyethylene mulch emissions alter soil metabolomes and microbial communities during field pea cultivation.

Findings

PE mulching increased bacterial abundance but suppressed fungal guilds like saprotrophic fungi and mycorrhizal symbionts.

PE-derived compounds correlated with organic nitrogen and lipids but negatively with benzenoids and nucleotides.

Soil metabolic pathways related to energy, lipid metabolism, and xenobiotic degradation were disrupted by PE mulch emissions.

Abstract

Background and Objectives: Polyethylene (PE) mulching enhances crop productivity through microclimate optimization but introduces synthetic polymer-derived compounds into agricultural soils. Despite widespread use, biochemical and microbial impacts of PE mulch emissions remain poorly understood. This study investigated the impact of PE mulch emissions on soil metabolomes and microbial communities during field pea (Pisum sativum L.) cultivation. Methods: A 75-day field experiment compared PE-mulched and non-mulched soils across five temporal sampling points (T0–T4). Headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry was used to identify PE-derived organic compounds in mulched soils. Microbial community structure was assessed through the phospholipids derived fatty acids (PLFA) approach, whereas mass spectrometric untargeted metabolomics was used to characterize the soil biochemical profiles. Results: Analysis identified 18 PE-derived organic compounds (n-alkanes, phthalates, and additives) in the mulched soils. PE mulching significantly increased bacterial abundance (anaerobic bacteria, actinomycetes, and aerobic bacteria) but suppressed all functional fungal guilds, particularly saprotrophic fungi (30% reduction) and arbuscular mycorrhizal symbionts. PE-derived organic compounds were associated primarily with the first RDA axis (RDA1), which alone explained 44.6% of the metabolome variance. These compounds presented strong positive correlations with organic nitrogen compounds and lipids and negative correlations with benzenoids and nucleotides. Pathway analysis revealed perturbations in energy metabolism, lipid metabolism, and xenobiotic degradation pathways. Conclusions: PE mulch emissions differentially shift soil microbial communities and metabolic networks, with bacterial proliferation contrasting with fungal suppression. These findings highlight the complex trade-offs between agronomic benefits and soil biological impacts, emphasizing the need for sustainable mulching alternatives.

Figures