# Evaluation of the inter-examiner reliability of myofascial trigger point identification in dogs

**Authors:** Bryce Talsma Roberts, Christina Montalbano, Felix Michael Duerr, Karolynn Mireya Ellis, Lindsay Hochman Elam

PMC · DOI: 10.3389/fvets.2026.1782274 · Frontiers in Veterinary Science · 2026-02-27

## TL;DR

This study evaluated how consistently two veterinarians can identify myofascial trigger points in dogs' hindlimbs, finding moderate agreement but low precision in their exact locations.

## Contribution

The first assessment of inter-examiner reliability for identifying myofascial trigger points in dogs.

## Key findings

- Examiners showed 81.4% agreement on MTrP presence or absence with a Cohen's kappa of 0.608, indicating moderate to substantial reliability.
- The mean distance between MTrP markings was 10.6 mm, suggesting low precision in localization.
- The cranial thigh muscle group had the highest number of identified MTrPs.

## Abstract

Myofascial trigger points (MTrPs) are a source of chronic pain in humans, but their diagnosis relies on subjective manual palpation. MTrP distribution has been described in dogs, but reliability between examiners remains unknown. This prospective, blinded, clinical investigation aimed to determine the inter-examiner reliability of MTrP identification in the hindlimb musculature of dogs and to describe their distribution.

Twenty-four geriatric and retired sled dogs were assessed for MTrPs in four hindlimb muscle groups (gluteals, cranial thigh, hamstrings, and medial thigh) by two veterinarians in random order. MTrP identification was based on previous methodology defining an MTrP as a distinct, hyperactive point or nodule eliciting a pain response, known as a jump sign. Examiners were blinded to each other's findings by marking MTrPs with invisible UV ink pens. Agreement on MTrP presence or absence in a muscle group, distance between examiners' points, and the number of MTrPs per muscle group were recorded. Inter-examiner reliability was assessed using Cohen's kappa.

In total, 380 MTrPs were identified by the two examiners across the 188 muscle groups assessed. Examiners demonstrated 81.4% agreement on MTrP presence or absence in a muscle group with a Cohen's kappa of 0.608 (95% CI: 0.491–0.724), indicating moderate to substantial inter-examiner reliability. However, the mean distance between examiners' markings for individual MTrPs was 10.6 ± 5.1 mm, suggesting a potential substantial lack of precision. The cranial thigh group contained the most MTrPs. Additionally, the more experienced examiner identified a greater number of MTrPs overall (p = 0.028).

This study provides the first assessment of inter-examiner reliability for MTrP identification in dogs. While examiners agreed on the general presence or absence of myofascial sensitivity within a muscle group, precise localization was unreliable. This lack of precision may be influenced by a number of factors, namely skin movement and the subjective nature of the assessment itself. This study highlights the need for further research and objective diagnostic tools for reliable, targeted MTrP identification.

## Full-text entities

- **Diseases:** chronic pain (MESH:D059350), pain (MESH:D010146)
- **Species:** Canis lupus familiaris (dog, subspecies) [taxon 9615], Homo sapiens (human, species) [taxon 9606]

## Full text

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## Figures

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## References

43 references — full list in the complete paper: https://tomesphere.com/paper/PMC13007605/full.md

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Source: https://tomesphere.com/paper/PMC13007605