# The Incorporation of Sorghum and Cowpea Protein Isolate Into Plant‐Based Burgers Improved Their Physicochemical and Sensory Properties

**Authors:** Andressa Alvarenga Silva, Valéria Aparecida Vieira Queiroz, Francielle Barbosa Pena, Cícero Beserra de Menezes, Mária Herminia Ferrari Felisberto, Renata Celi Lopes Toledo, Barbara Pereira da Silva, Hércia Stampini Duarte Martino

PMC · DOI: 10.1111/1750-3841.70953 · Journal of Food Science · 2026-03-22

## TL;DR

Adding sorghum and cowpea protein to plant-based burgers improves their taste, texture, and nutrition compared to soy-based burgers.

## Contribution

Sorghum and cowpea protein isolates are shown to enhance the sensory and nutritional properties of plant-based burgers.

## Key findings

- Burgers with tannin-rich sorghum had higher phenolics, anthocyanins, and antioxidant capacity.
- Sorghum-based burgers provided high levels of protein, fiber, iron, and zinc.
- Tannin-rich formulations showed mechanical resistance and lower pH.

## Abstract

This study aimed to evaluate the consumer acceptance test, purchase intention, and physicochemical and technological properties of cooked plant‐based burgers formulated with sorghum and cowpea protein isolate, compared with a commercial burger (CO). Five formulations were tested: F1 (tannin‐free sorghum, 19% protein), F2 (tannin‐rich sorghum, 19%), F3 (tannin‐free sorghum, 24%), F4 (tannin‐rich sorghum, 24%), and CO (commercial hamburger made from soy). No differences were observed between sample F1 and the CO regarding color preference, flavor, texture, and overall impression. Sample F2 was selected for further analysis due to its equivalent protein content and the presence of tannins in its composition, as well as exhibiting aroma, flavor, and overall impression similar to CO. Following sensory screening, only the two most accepted formulations were selected for physicochemical and technological characterization. F1 and F2 showed excellent sources of protein, dietary fiber, iron, and zinc. F2 presented the highest tannins, p‐coumaric and ferulic acids, 7‐methoxyapigeninidin, and antioxidant capacity, with lower pH and higher mechanical resistance. Burgers formulated with sorghum and cowpea protein isolate proved to be promising for the development of meat analogues, with the presence of anthocyanins and phenolic acids contributing to the improvement of nutritional, technological, and sensory properties.

Sorghum and cowpea protein isolates are sustainable alternatives for meat analogues. Tannin‐rich sorghum burgers showed higher phenolics, anthocyanins, and antioxidant capacity. Sorghum‐based burgers provided high protein, fiber, iron, and zinc contents.

## Linked entities

- **Chemicals:** p-coumaric acid (PubChem CID 637542), ferulic acid (PubChem CID 445858), 7-methoxyapigeninidin (PubChem CID 76308630)

## Full-text entities

- **Diseases:** asthma (MESH:D001249), allergic (MESH:D004342), anaphylactic shock (MESH:D000707), WHC (MESH:D000069578), weight loss (MESH:D015431)
- **Chemicals:** apigeninidin (MESH:C104027), Carbohydrate (MESH:D002241), Trolox (MESH:C010643), flavonoids (MESH:D005419), HCl (MESH:D006851), Mineral (MESH:D008903), nitrogen (MESH:D009584), formic acid (MESH:C030544), Cu (MESH:D003300), CE (MESH:D002563), p-coumaric acid (MESH:C495469), Water (MESH:D014867), potassium persulfate (MESH:C009007), xanthan gum (MESH:C002563), F2 (MESH:D005461), Condensed tannins (MESH:D044945), eriodictyol (MESH:C007619), acetonitrile (MESH:C032159), ethyl acetate (MESH:C007650), fiber (MESH:D004043), methanol (MESH:D000432), oil (MESH:D009821), salt (MESH:D012492), soybean oil (MESH:D013024), EC (MESH:D002392), acetic acid (MESH:D019342), Fe (MESH:D007501), Tannin (MESH:D013634), luteolinidin (MESH:C518537), Lipid (MESH:D008055), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (MESH:C002502), NaOH (MESH:D012972), Zn (MESH:D015032), 3-Desoxyanthocyanidins (-), PTFE (MESH:D011138), 7-methoxy apigeninidin (MESH:C104028), Phenolic Acid (MESH:C017616), hydrogen (MESH:D006859), Mn (MESH:D008345), nitric acid (MESH:D017942), ferulic acid (MESH:C004999), acetone (MESH:D000096), Anthocyanin (MESH:D000872), isorhamnetin 3-O-rutinoside (MESH:C515815), ethanol (MESH:D000431)
- **Species:** Sorghum bicolor (broomcorn, species) [taxon 4558], Allium sativum (garlic, species) [taxon 4682], Homo sapiens (human, species) [taxon 9606], Allium cepa (onion, species) [taxon 4679], Powellomyces sp. EA (species) [taxon 252690], Oryza sativa (Asian cultivated rice, species) [taxon 4530], Glycine max (soybean, species) [taxon 3847], Vigna unguiculata (cowpea, species) [taxon 3917]
- **Mutations:** Q320 M

## Full text

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## Figures

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## References

60 references — full list in the complete paper: https://tomesphere.com/paper/PMC13006479/full.md

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Source: https://tomesphere.com/paper/PMC13006479