# Multi-carbohydrase complex supplementation improves crude protein and energy utilization of corn-soybean-based diets with or without HP-DDGS in growing pigs

**Authors:** Stephane Alverina Briguente da Motta, Wagner Azis Garcia de Araújo, Naiara Simarro Fagundes, Adriana Berti Toscan, Afonso Luna Miranda, Alejandra Gutierrez Riaño, Giovana Thais Soares Pereira, Raphaela Ribeiro Neves, Geovana da Silva Ribeiro, Bruno Alexander Nunes Silva

PMC · DOI: 10.1093/tas/txag025 · Translational Animal Science · 2026-02-28

## TL;DR

Adding a multi-carbohydrase complex to pig diets improves protein and energy digestion, even when using corn-soybean or high-protein corn by-products.

## Contribution

Demonstrates that multi-carbohydrase complex improves nutrient utilization in diets with or without high-protein corn by-products in pigs.

## Key findings

- HP-DDGS reduced digestibility of dry matter, crude protein, and energy compared to corn-soybean diets.
- MCC supplementation improved nutrient and energy digestibility regardless of diet composition.
- MCC can enhance the use of ethanol by-products in swine nutrition.

## Abstract

The use of high protein corn dried distillers’ grains with solubles (HP-DDGS) presents distinct nutritional and economic value compared to corn and soybean meal. This co-product is characterized by a high concentration of non-starch polysaccharides. The hypothesis of this study was that the inclusion of a multi-carbohydrase complex (MCC) in a corn-soybean based diet with or without HP-DDGS would improve nitrogen and energy digestibility and utilization in growing pigs. A total of 8 pigs were used in a digestibility study. Animals were assigned to 4 dietary treatments using a replicated 4 × 4 Latin square design. Experimental diets consisted of two basal diets: a corn–soybean meal diet and a corn–soybean meal diet containing 20% HP-DDGS. Each basal diet was formulated with or without the inclusion of MCC. Data were analyzed using an ANOVA with HP-DDGS inclusion, enzyme inclusion, animal, and period as the main effects. Means were then compared by the Student Newman-keuls test. The inclusion of HP-DDGS reduced (P < 0.001) the apparent total tract digestibility of dry matter, crude protein, gross energy, energy metabolizability coefficient, and decreased digestible energy and metabolizable energy compared to the corn-soybean diet. Conversely, MCC supplementation improved (P < 0.05) nutrient and energy digestibility, regardless of diet composition. The present study demonstrated that the use of a MCC in growing pig diets has the potential to improve nutrient digestibility and utilization not only when HP-DDGS is used, but also in regular corn-soybean based diets.

Graphical Abstract

The inclusion of a multi-carbohydrase complex in pig diets significantly improved nutrient digestibility in both conventional corn- and soybean-based diets and HP-DDGS–containing diets, highlighting the importance of enzyme supplementation. These results indicate that multi-carbohydrase complex is a promising strategy to optimize nutrient utilization and enable the sustainable and cost-effective inclusion of ethanol by-products in swine nutrition.

## Linked entities

- **Species:** Sus scrofa (taxon 9823)

## Full-text entities

- **Chemicals:** DDGS (-), polysaccharides (MESH:D011134), starch (MESH:D013213), nitrogen (MESH:D009584)
- **Species:** Glycine max (soybean, species) [taxon 3847], Sus scrofa (pig, species) [taxon 9823]

## Full text

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## Figures

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## References

22 references — full list in the complete paper: https://tomesphere.com/paper/PMC13005691/full.md

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Source: https://tomesphere.com/paper/PMC13005691