# Complete Genome Annotation of Mycobacteriophage Kremtemulon

**Authors:** Cole R. Jirsa, Bethany M. Wise, Kaylia Edwards, Jerusalem Mussie, Owen Tolbert, Danielle M. Heller

PMC · DOI: 10.17912/micropub.biology.001985 · microPublication Biology · 2026-03-06

## TL;DR

This paper presents the full genome analysis of a temperate phage called Kremtemulon, which infects Mycobacterium smegmatis.

## Contribution

The study provides a complete genome annotation and classification of the mycobacteriophage Kremtemulon into Cluster A, Subcluster A4.

## Key findings

- Kremtemulon has a 51,438 bp genome encoding 87 putative genes, 36 with predicted functions.
- The phage is classified into Cluster A, Subcluster A4 based on gene content.
- Kremtemulon is identified as a temperate phage due to its turbid plaques and presence of an immunity repressor and tyrosine integrase.

## Abstract

Kremtemulon is a siphovirus isolated on the host bacterium
Mycobacterium smegmatis 
mc
2
155 with a genome spanning 51,438 bp in length. Kremtemulon encodes 87 putative genes, 36 of which have predicted functions, and based on gene content, is assigned to Cluster A and Subcluster A4. Kremtemulon forms turbid plaques and encodes for both an immunity repressor and a tyrosine integrase, suggesting that Kremtemulon is a temperate phage.

## Full-text entities

- **Species:** Mycolicibacterium smegmatis MC2 155 (strain) [taxon 246196]

## Full text

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## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC13005150/full.md

## References

11 references — full list in the complete paper: https://tomesphere.com/paper/PMC13005150/full.md

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Source: https://tomesphere.com/paper/PMC13005150