Spontaneous aging-associated inflammation and genome instability in the immune system of turquoise killifish
Gabriele Morabito, Handan Melike Dönertas, Luca Sperti, Jens Seidel, Aysan Poursadegh Zonouzi, Michael Poeschla, Dario Riccardo Valenzano
TL;DR
Turquoise killifish show rapid immune system aging with inflammation and DNA damage, offering a model for studying and targeting aging interventions.
Contribution
The study reveals age-related immune system changes in turquoise killifish, including inflammation and genome instability.
Findings
Old killifish show increased inflammation markers in their immune system.
Immune progenitors from old killifish display elevated DNA damage markers.
Killifish exhibit immune system aging within less than 10 weeks.
Abstract
Turquoise killifish (Nothobranchius furzeri) are naturally short-lived vertebrates that recapitulate key aspects of human aging. However, the molecular and cellular causes of systemic aging in killifish are poorly understood. Here we ask whether killifish undergo age-dependent changes in the main hematopoietic organ (kidney marrow), which may contribute to systemic aging. To characterize immune aging in killifish, we used single-cell RNA sequencing, cytometry and functional in vitro assays on kidney marrow cells from young-adult and old killifish, together with proteomic profiling of both kidney marrow-derived cells and plasma. We show that old killifish display increased markers of inflammation; while immune progenitor-like cell clusters from adult killifish display markers of active proliferation and replication-independent DNA repair, immune cell progenitors from old killifish…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16
Figure 17
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsFish biology, ecology, and behavior · Indigenous Health and Education · Zebrafish Biomedical Research Applications