Genetic, epigenetic and metabolite variation in peripheral European Yew (Taxus baccata L.) populations at an unexplored part of the species natural distribution
Eleftheria Dalmaris, Evangelia Avramidou, Eirini Sarrou, Aliki Xanthopoulou, Salvatore Multari, Stefan Martens, Filippos A. Aravanopoulos
TL;DR
This study explores genetic, epigenetic, and chemical diversity in peripheral Greek yew populations to assess their potential for sustainable taxane production.
Contribution
The study identifies peripheral Greek yew populations as valuable genetic resources for taxane production and conservation.
Findings
Taxane concentrations varied significantly among populations and seasons, with 10-deacetylbacatin III as the dominant compound.
Genetic diversity was substantial with significant population differentiation, while epigenetic diversity was moderate.
Multivariate analyses revealed clear genetic and metabolomic population structuring, but less so for epigenetic variation.
Abstract
Taxanes form effective anticancer agents, which are found in the leaves and bark of the yew tree (Taxus L.). Paclitaxel (Taxol®) and related taxanes are widely used in cancer therapy. Due to the high demand of taxanes, there is strong pharmaceutical interest in evaluating unexplored population diversity as a potential genetic and biochemical resource. Three peripheral Greek Taxus baccata L. populations (Mt Cholomon, Mt Olympus and Mt Vourinos) were investigated to assess genetic (microsatellite markers), epigenetic (methylation sensitive amplified markers) and chemodiversity (targeted LC-MS/MS analysis of five major taxanes) variation. Taxane concentration varied significantly among populations and seasons. The dominant compound in needles was 10-deacetylbacatin III (DAB), ranging from 267.8 (Mt Vourinos) to 517.6 (Mt Olympus) mg kg-1 dw. Substantial genetic diversity (AR = 5.00; He =…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsCancer Treatment and Pharmacology · Carcinogens and Genotoxicity Assessment · DNA Repair Mechanisms