Systematic identification of variant-specific RNA structure-small molecule interactions exemplified by RNA G-quadruplexes

TL;DR

This paper introduces a new method to detect how genetic mutations in RNA affect small molecule binding, focusing on RNA structures called G-quadruplexes.

Contribution

The novel contribution is the development of BIVID-MaP, a high-throughput method for identifying variant-specific RNA-small molecule interactions.

Findings

BIVID-MaP successfully identifies interactions between RNA G-quadruplexes and small molecules affected by single-nucleotide variants.

Cancer-associated mutations can significantly alter small molecule binding intensity by modifying RNA G-quadruplex structures.

Abstract

Individual genetic variations, such as cancer-associated somatic mutations, alter RNA structures, thereby potentially enhancing or inhibiting the binding of RNA-targeting small molecules. However, to date, no approach has been available to identify these variant-specific RNA-small molecule interactions due to technical limitations. Here, we present Binding- and Vinyl-Quinazolinone-Induced Deletion-Based Mutational Profiling (BIVID-MaP), a high-throughput method for detecting RNA-small molecule interactions that combines binding-dependent covalent modification with profiling of deletions upon reverse transcription via deep sequencing. Using BIVID-MaP, we uncovered numerous variant-specific interactions between a G-quadruplex (G4)-binding small molecule and RNAs harboring single-nucleotide variants. Several cancer-associated somatic mutations significantly influence the binding intensity of a small molecule by affecting target G4 structures. These results demonstrate that BIVID-MaP can reveal previously ignored variant-specific RNA-small molecule interactions affected only by a single-nucleotide mutation, which may contribute to the development of RNA-targeting drugs in the future.

Cancer-associated somatic mutations can change RNA structures. Here, the authors develop a method to detect interactions between RNA and small molecules, showing that such mutations can affect small molecule binding by changing the RNA’s G quadruplex structures.

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