# Aeroponic cultivation of lettuce: Unravelling varietal performance and trait interrelationships for enhanced productivity

**Authors:** Anand Sahil, S. R. Singh, Lalu Prasad Yadav, A. K. Singh, Vikas Yadav, Sanjay Kumar, N. K. Meena, Prashant Kaushik, Prakash Mahala, Eugenio Llorens, Eugenio Llorens, Eugenio Llorens

PMC · DOI: 10.1371/journal.pone.0345258 · PLOS One · 2026-03-19

## TL;DR

This study compares five lettuce varieties grown in aeroponic systems to determine which ones perform best and how traits like leaf weight and chlorophyll content relate to productivity.

## Contribution

The study introduces aeroponic cultivar performance analysis with a focus on trait interrelationships using PCA and correlation methods.

## Key findings

- ‘Bingo’ had the highest total leaf weight, while ‘Summer Star’ showed the best yield per hectare.
- PCA revealed that leaf morphology and chlorophyll concentration explain most of the variance in aeroponic lettuce growth.
- Yield negatively correlated with total chlorophyll content, while plant height and leaf area positively correlated.

## Abstract

Aeroponic systems offer a sustainable and efficient platform for cultivating high-quality leafy greens, such as lettuce (Lactuca sativa L.). This study investigated the performance of five distinct lettuce cultivars (‘Summer Star’, ‘Grand Rapid’, ‘Tango’, ‘Bingo’, and ‘Black Rose’) within a controlled aeroponic environment to identify superior varieties and elucidate the intricate relationships among key agronomic traits. A comprehensive suite of statistical analyses was employed, including Analysis of Variance (ANOVA), Pearson correlation analysis, Principal Component Analysis (PCA), and genetic variability assessment using GCV and PCV. Results revealed significant differences among cultivars for growth parameters, yield components, and quality attributes. Notably, ‘Bingo’ exhibited the highest total leaf weight, while ‘Summer Star’ demonstrated superior yield per hectare. Correlation analysis highlighted strong positive associations between plant height, leaf area index, and chlorophyll content, whereas yield exhibited a negative correlation with total chlorophyll content. PCA identified key underlying factors contributing to the observed variation, with the first two principal components explaining 86.13% of the total variance, underscoring the importance of leaf morphology and chlorophyll concentration in driving aeroponic growth and productivity. The findings underscore the potential of aeroponics to contribute to global food security by enhancing the productivity and quality of leafy greens.

## Full-text entities

- **Genes:** PCSK1 (proprotein convertase subtilisin/kexin type 1) [NCBI Gene 5122] {aka BMIQ12, NEC1, PC1, PC1/3, PC3, SPC3}
- **Diseases:** cardiovascular ailments (MESH:D002318), diabetes (MESH:D003920), inflammation (MESH:D007249), diseases (MESH:D004194), obesity (MESH:D009765), cancer (MESH:D009369), MS (MESH:D009103), malnutrition (MESH:D044342)
- **Chemicals:** TC (MESH:D013667), Ascorbic acid (MESH:D001205), CO2 (MESH:D002245), polyphenols (MESH:D059808), petroleum ether (MESH:C004544), Chlorophyll (MESH:D002734), carotenoid (MESH:D002338), water (MESH:D014867), silver (MESH:D012834), sodium (MESH:D012964), nitric oxide (MESH:D009569), Chyll (-), folate (MESH:D005492), reactive oxygen species (MESH:D017382), polyethylene (MESH:D020959), oxygen (MESH:D010100), hydrogen peroxide (MESH:D006861), Chlorophyll 'b' (MESH:C037184), acetone (MESH:D000096), anthocyanin (MESH:D000872), 2,6-dichlorophenol-indophenol (MESH:D015086)
- **Species:** Cucumis sativus (cucumber, species) [taxon 3659], Homo sapiens (human, species) [taxon 9606], Solanum lycopersicum (tomato, species) [taxon 4081], Lactuca sativa (cultivated lettuce, species) [taxon 4236], Peanut clump virus (no rank) [taxon 28355], Mus musculus (house mouse, species) [taxon 10090], Solanum tuberosum (potatoes, species) [taxon 4113]

## Full text

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## Figures

7 figures with captions in the complete paper: https://tomesphere.com/paper/PMC13001950/full.md

## References

52 references — full list in the complete paper: https://tomesphere.com/paper/PMC13001950/full.md

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Source: https://tomesphere.com/paper/PMC13001950