Resolving the glycosaminoglycan signature of ischemic stroke brain using PRM-based IR-MALDESI mass spectrometry imaging
Tana V. Palomino, Noah Campbell, Yunxin Ouyang, Nidhi Naik, Adam M. Hawkridge, Tatiana Segura, David C. Muddiman
TL;DR
This study uses a new mass spectrometry technique to identify changes in glycosaminoglycans in ischemic stroke brains, revealing consistent patterns of specific isomers.
Contribution
The study introduces IR-MALDESI with PRM to resolve labile CS-GAG isomers in stroke tissue without chemical derivatization.
Findings
Both mono-sulfated CS-GAG positional isomers were upregulated in ischemic stroke brain tissue.
The relative abundance of the isomers remained constant across the tissue.
IR-MALDESI with PRM enables accurate characterization of intact CS-GAG isomers.
Abstract
Stroke is the second most common cause of death in the world and a leading cause of disability. Ischemic stroke is the most common type of stroke (~87%), necessitating research into effective treatments. Chondroitin sulfate (CS) is a sulfated glycosaminoglycan (GAG) found in the central nervous system (CNS) that contains labile sulfate groups which, upon loss, leads to inaccurate structural annotations. Variable sulfation patterns have been implicated in several neurological diseases. Additionally, CS-GAG analysis is challenging due to labile sulfate groups and the presence of positional isomers. These isomers must be distinguished to develop effective targeted therapies. Currently, glycan mass spectrometry imaging (MSI) lacks soft ionization sources which impedes intact analysis of the labile sulfate modifications. Infrared matrix-assisted laser desorption electrospray ionization…
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Taxonomy
TopicsProteoglycans and glycosaminoglycans research · Mass Spectrometry Techniques and Applications · Glycosylation and Glycoproteins Research