Decoding the functional role of the calcium ATPase YloB in microbially induced calcite precipitation and sporulation in Solibacillus silvestris

TL;DR

The calcium ATPase YloB in Solibacillus silvestris is not involved in calcium detoxification or calcite precipitation but is crucial for spore maturation by transporting calcium into the forespore.

Contribution

This study reveals a novel role for a bacterial calcium ATPase in sporulation rather than calcium detoxification or biomineralization.

Findings

Deletion of yloB does not affect calcium detoxification or calcite precipitation.

YloB is essential for Ca-DPA accumulation and spore maturation.

YloB localizes to the cell membrane surrounding the forespore during sporulation.

Abstract

YloB in Solibacillus silvestris is not involved in calcium detoxification or biomineralization but is important for sporulation, likely transporting Ca²⁺ into the forespore for Ca-DPA accumulation and spore maturation.

Calcium homeostasis is essential for bacterial physiology, yet its regulation and role in specialized processes such as microbially induced calcite precipitation (MICP) and sporulation remain poorly understood. Here, we characterized the sole annotated P-type calcium ATPase in Solibacillus silvestris, which was previously shown to be upregulated under MICP conditions. We named the protein YloB based on homology to the Bacillus subtilis protein. Structural modelling confirmed that YloB possesses the conserved domains and motifs typical of calcium-binding P-type ATPases. Deletion of yloB did not affect growth under elevated calcium levels, nor did it reduce MICP yields, indicating that YloB is dispensable for calcium detoxification and biomineralization. In contrast, ∆yloB cells produced only partially dehydrated spores with severely reduced heat resistance, a defect rescued by complementation. A YloB-mNeonGreen fluorescent fusion localized specifically to the cell membrane surrounding the forespore during early engulfment and was retained at this site throughout the remainder of sporulation. A transcriptional PyloB-msfGFP reporter demonstrated that yloB transcription was induced in the early stages of sporulation but did not respond to a calcium stimulus. These results show that YloB does not act to detoxify calcium from the cytoplasm of S. silvestris, but rather functions to pump calcium into the forespore, enabling Ca-dipicolinic acid accumulation and proper spore maturation. Our study reveals a specialized role for a bacterial calcium ATPase in sporulation, distinguishing calcium transport for endospore development from biomineralization and global calcium homeostasis.

Figures