Preparation and Characterization of Alginate‐Based Bioinks for Three‐Dimensional Bioprinting of Cell‐Laden Constructs
Nuraina Anisa Dahlan, Farinaz Ketabat, Kathryn Avery, Xavier L. Tabil, Samira Khoz, Elise Altarriba, Neeraj Dhar, Xiongbiao Chen
TL;DR
This paper provides detailed protocols for preparing and characterizing alginate-based bioinks used in 3D bioprinting of cell-laden constructs for tissue engineering and disease modeling.
Contribution
The study introduces standardized, reproducible methods for preparing and evaluating alginate-based bioinks and bioprinted constructs.
Findings
Protocols for bioink preparation, rheological characterization, and bioprinting are presented.
The methods include post-printing culture techniques and assays for printability and cell viability.
Abstract
Biomaterial‐based bioinks are increasingly utilized in bioprinting to engineer three‐dimensional (3D) constructs with living cells for tissue engineering and disease modeling. Among various bioinks explored, alginate‐based formulations stand out due to their good biocompatibility, mild gelation conditions, tunable mechanical properties, and ease of crosslinking via divalent cations such as calcium. Despite their widespread use, standardized protocols for preparing alginate‐based bioinks and characterizing bioprinted constructs have not been well documented. Our laboratory has developed and validated reproducible methods for preparing a variety of alginate‐based bioinks and printing cell‐laden constructs tailored for diverse applications. In this article, we present detailed step‐by‐step protocols covering bioink preparation and rheological characterization, extrusion‐based bioprinting…
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Taxonomy
Topics3D Printing in Biomedical Research · Cellular Mechanics and Interactions · Pluripotent Stem Cells Research