# AI-driven label-free Raman spectromics for intraoperative spinal tumor assessment

## TL;DR

A new AI system called SpineXtract enables rapid spinal tumor diagnosis during surgery using Raman spectromics, offering faster and more accurate results than current methods.

## Contribution

The first AI-powered system for intraoperative spinal tumor classification using label-free Raman histology.

## Key findings

- SpineXtract achieved 92.9% macro-average balanced accuracy in classifying spinal tumors within 5 minutes.
- The system outperformed existing brain tumor classifiers by 15.6% in accuracy.
- Performance remained consistent across multiple institutions and tumor types.

## Abstract

Spinal tumor surgery requires rapid tissue diagnosis to guide surgical decisions and further treatment strategies, yet current intraoperative methods are time-intensive and require specialized expertise. No AI systems exist for real-time spinal tumor classification during surgery. We developed SpineXtract, the first AI-powered system for rapid intraoperative spinal tumor diagnosis using stimulated Raman histology (SRH) — a label-free Raman spectromics imaging technique without tissue processing available during surgery. We created a transformer-based classifier optimized for spinal tissue characteristics to identify common tumor types: meningioma, schwannoma, ependymoma, and metastasis. The system was tested in an international, multicenter, simulated, single-arm study using existing SRH datasets (44 patients, 142 slide-images) from three international institutions, with final pathological diagnosis as reference standard. SpineXtract achieved a 92.9% macro-average balanced accuracy (95% CI: 85.5–98.2) within 5 minutes (tumor-specific accuracy range, 84.2–98.6%), while providing quantitative microscopic feedback for granular tissue analysis. Performance remained consistent across institutions (macro balanced accuracy 91.4–92.0%) and outperformed existing brain tumor classifiers by 15.6%. Our results demonstrate clinical applicability, enabling rapid intraoperative diagnosis with performance exceeding current methods, potentially transforming intraoperative diagnostic workflows in spinal tumor surgery.