# Reply to Chen: Methodological and analytical considerations in the study of H3-Dendra2 distribution

**Authors:** Anqi Li, Dong Tong, Benjamin Ohlstein, Zheng Guo

PMC · DOI: 10.1073/pnas.2536993123 · Proceedings of the National Academy of Sciences of the United States of America · 2026-03-06

## Full-text entities

- **Genes:** His2Av (Histone H2A variant) [NCBI Gene 43229] {aka *i H2av, 5499, CG5499, Dmel\CG5499, H2A, H2A.F/Z}, His3.3B (Histone H3.3B) [NCBI Gene 31848] {aka CG8989, Dmel\CG8989, H3.3, H3.3B, His 3.3 B, His 3.3B}, His3:CG33860 (histone H3) [NCBI Gene 3772517] {aka CG33860, Dmel\CG33860}, esg (escargot) [NCBI Gene 34903] {aka 4B7, BG01042, BG:DS07851.7, CG3758, Dmel\CG3758, br43}
- **Chemicals:** Alexa Fluor 647 (MESH:C569686), Dendra2 (-)
- **Species:** Drosophila melanogaster (fruit fly, species) [taxon 7227]

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## References

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Source: https://tomesphere.com/paper/PMC12993950