# When collagen fails: Zinc isotopes unlock Sumerian lifeways in southern Mesopotamia

## TL;DR

Zinc isotopes help reveal ancient diets in southern Mesopotamia where collagen preservation was poor, showing people ate C3 cereals, terrestrial animals, and freshwater resources.

## Contribution

Zinc isotope analysis is introduced as a novel method to reconstruct diets in collagen-poor archaeological contexts.

## Key findings

- Ancient diets included C3 cereals, terrestrial animal protein, and freshwater resources but no marine fish.
- Zinc isotopes proved effective in identifying animal feeding practices and early-life nutrition.
- Dietary patterns were consistent across sexes, indicating similar food access in a nonelite population.

## Abstract

Understanding ancient diets is one of the keys to reconstructing lifeways and social structures. In what are now arid regions like southern Mesopotamia, poor collagen preservation has long hindered direct dietary reconstructions. Here, we apply zinc isotope analysis to human and faunal dental enamel from the third-millennium BCE site of Abu Tbeirah (southern Iraq), offering a method to overcome this limitation. Combined with carbon and oxygen isotopes and trace element ratios (Ba/Ca and Sr/Ca), zinc isotopes reveal an omnivorous diet based on C3 cereals, terrestrial animal protein, and possibly freshwater resources, with no evidence of marine fish consumption. These findings offer individual-level insight into subsistence practices, early-life nutrition, and animal management within a nonelite population in early-urbanized southern Mesopotamia.

Reconstructing past lifeways and diets is essential to understanding the emergence of urban societies. However, in what are now arid environments like southern Mesopotamia, poor collagen preservation has long hampered direct isotopic analysis of trophic levels. This limitation has left key gaps in our understanding of subsistence in one of the world’s earliest urban heartlands. Here, we apply zinc isotope analysis to human and faunal dental enamel from the third-millennium BCE site of Abu Tbeirah (Iraq), integrating δ13C, δ18O, and trace element ratios (Ba/Ca and Sr/Ca). This multiproxy approach reveals an omnivorous diet based on C3 cereals, terrestrial animal products (likely including pigs), and limited freshwater resources, with no or little evidence of marine fish consumption, despite the site’s proximity to the ancient shoreline. Dietary patterns do not vary by sex, suggesting broad access to similar food sources within this nonelite population. Moreover, zinc and carbon isotopes proved valuable in identifying animal feeding practices. Our results provide direct dietary evidence from southern Mesopotamia, overcoming long-standing preservation challenges. The results allow us to evaluate specific expectations about diet and animal management in a collagen-poor context, also highlighting early-life feeding behaviors. They demonstrate the power of zinc isotopes to reconstruct trophic level in collagen-poor contexts, opening broad avenues for bioarchaeological research in early complex societies.