# Protocol for the synthesis and characterization of Ru@PCN@PEI nanoparticles with superoxide dismutase catalytic activity

## TL;DR

This paper provides a detailed protocol for making Ru@PCN@PEI nanoparticles with superoxide dismutase-like activity for biomedical and catalytic uses.

## Contribution

A new synthesis and characterization protocol for Ru@PCN@PEI nanoparticles with SOD-like catalytic activity is presented.

## Key findings

- The protocol includes steps for size regulation of PCN-222(Mn) nanoparticles using ZrOCl2:BA ratio.
- Ru@PCN@PEI nanoparticles were characterized using TEM, SEM, and DLS for morphology.
- SOD-like activity was evaluated using UV-vis spectroscopy.

## Abstract

Bimetallic organic frameworks (BMOFs) possess versatile structural and functional features that enable applications in catalysis and biomedicine. Here, we present a protocol to synthesize Ru@PCN@PEI nanoparticles by loading Ru nanoparticles onto PCN-222(Mn) and coating the surface with polyethyleneimine (PEI). We describe steps for precursor preparation, particle size regulation, and surface modification. We detail procedures for morphological characterization by transmission electron microscopy and assessment of catalytic activity using ultraviolet-visible (UV-vis) spectroscopy. This protocol enables the preparation and functionalization of BMOFs for advanced nanobiomaterial applications.

•Optimize PCN-222(Mn) NP size by adjusting the ZrOCl2:BA ratio•Steps for synthesizing Ru@PCN@PEI NPs by loading Ru and coating with PEI•Guidance on the characterization of Ru@PCN@PEI NPs using TEM, SEM, and DLS•Procedures for evaluating SOD-like activity of Ru@PCN@PEI NPs via UV-vis

Optimize PCN-222(Mn) NP size by adjusting the ZrOCl2:BA ratio

Steps for synthesizing Ru@PCN@PEI NPs by loading Ru and coating with PEI

Guidance on the characterization of Ru@PCN@PEI NPs using TEM, SEM, and DLS

Procedures for evaluating SOD-like activity of Ru@PCN@PEI NPs via UV-vis

Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Bimetallic organic frameworks (BMOFs) possess versatile structural and functional features that enable applications in catalysis and biomedicine. Here, we present a protocol to synthesize Ru@PCN@PEI nanoparticles by loading Ru nanoparticles onto PCN-222(Mn) and coating the surface with polyethyleneimine (PEI). We describe steps for precursor preparation, particle size regulation, and surface modification. We detail procedures for morphological characterization by transmission electron microscopy and assessment of catalytic activity using ultraviolet-visible (UV-vis) spectroscopy. This protocol enables the preparation and functionalization of BMOFs for advanced nanobiomaterial applications.