# PIEZO1 Channels Modulate the Small Extracellular Vesicle Release in C2C12 Cells

## TL;DR

PIEZO1 channels influence the release of small extracellular vesicles in muscle cells, with different effects in myoblasts and myotubes.

## Contribution

This study reveals a novel role of PIEZO1 in modulating extracellular vesicle release in myogenic cells.

## Key findings

- PIEZO1 activation increased extracellular vesicle release in myotubes but not in myoblasts.
- Vesicles from both cell types promoted myotube formation but with different capacities.
- PIEZO1 activation increased PIEZO1 protein expression in vesicles from myotubes.

## Abstract

PIEZO1 are mechanically‐activated ion channels expressed in many cell types. Their pharmacological activation by the selective agonist Yoda1 has been reported to favor skeletal muscle regeneration by controlling the fate of myogenic precursors cells, but the underlying mechanisms remain largely unknown. Hereby, we investigated the possibility that PIEZO1 could control the release of small extracellular vesicles in myogenic C2C12 cells. Myoblasts and differentiated myotubes were treated with the PIEZO1 agonist Yoda1 (5 μM) for 24 hours. Released small extracellular vesicles were isolated by ultracentrifugation methods, and characterized by Western blotting, Nano Tracking and proteomic analysis. Pharmacological activation of PIEZO1 showed cell‐type‐specific effects: In myoblasts, Yoda1 treatment did not significantly affect the size or release of the small extracellular vesicles and resulted in only minor alterations to their proteomic profile. In myotubes Yoda1 treatment significantly increased small extracellular vesicles release and caused subtsantial alterations to the proteomic cargo. Notably, small extracellular vesicles released from both myoblasts and myotubes under PIEZO1 activation promoted myotube formation, though they did so through different capacities. Interestingly, in myotubes, Yoda1 also increased the expression of PIEZO1 protein of the vesicles suggesting a different biogenesis in undifferentiated and differentiated myogenic cells. Here, we propose PIEZO1 as a key element in controlling the release of small extracellular vesicles in myogenic precursors. Given the critical role of small extracellular vesicles in intercellular communication during muscle regeneration, our findings contribute to a better understanding of the role of PIEZO1 in the physiopathology of skeletal muscle tissue.