# The genome sequence of the Carline Skipper, Pyrgus carlinae (Rambur, 1839) (Lepidoptera: Hesperiidae)

## TL;DR

This paper presents the genome sequence of the Carline Skipper butterfly, including detailed assembly and gene annotation.

## Contribution

The study provides a high-quality genome assembly and gene annotation for Pyrgus carlinae as part of a larger butterfly and moth genome initiative.

## Key findings

- The genome assembly includes two haplotypes with lengths of 808.07 and 554.08 megabases.
- Haplotype 1 is scaffolded into 25 chromosomal pseudomolecules, including sex chromosomes W and Z.
- Gene annotation identified 14,216 protein-coding genes using Ensembl.

## Abstract

We present a genome assembly from a female specimen of
Pyrgus carlinae (Carline Skipper; Arthropoda; Insecta; Lepidoptera; Hesperiidae). The assembly contains two haplotypes with total lengths of 808.07 megabases and 554.08 megabases. Most of haplotype 1 (99.18%) is scaffolded into 25 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.43 kilobases. Gene annotation of this assembly on Ensembl identified 14 216 protein-coding genes. This work is part of Project Psyche, a collaborative programme generating genomes for European butterflies and moths.