# Genotype by environment interaction and yield stability analysis of durum wheat (Triticum turgidum var. durum) varieties using AMMI and GGE biplot analysis in Amahara Region, Northwest Ethiopia

**Authors:** Alemnesh Eskezia, Alamir Ayenew, Yohannes Azene, Mulugeta Aytenew, Kelemu Nakachew, Mehdi Rahimi, Mehdi Rahimi, Mehdi Rahimi, Mehdi Rahimi

PMC · DOI: 10.1371/journal.pone.0342401 · PLOS One · 2026-03-13

## TL;DR

This study evaluates the performance and stability of durum wheat varieties in Ethiopia, identifying the best variety for local farmers to improve yields.

## Contribution

The study identifies Tesfaye as a stable and high-yielding durum wheat variety suitable for the Amhara Region.

## Key findings

- Tesfaye had the highest mean yield of 3.74 t ha-1 across tested environments.
- Environmental factors contributed 45% of the variation in yield performance.
- AMMI and GGE biplot analyses identified Tesfaye and Don metteo as stable genotypes.

## Abstract

Durum wheat is a staple crop for over one- third of the global population; however, its production in Ethiopia, particularly in the Amhara Region, faces major challenges due to limited adaptable varieties, poor management practices, and environmental constraints. Newly released wheat varieties often fail in unfamiliar environments, leading to yield losses. To address this issue, a study was conducted to evaluate the adaptability, performance, and yield stability of recently released high-performing durum wheat cultivars in the Amahara region. The field trials were conducted over two consecutive years (2022/2023 and 2023/2024) at three districts; Simada, Bichena, and Dabat, using a Randomized Complete Block Design with three replications. Data were collected on main agronomic traits, including growth, phenological, and yield traits such as grain yield. Statistical analyses included SAS 9.4 and R software were used, showing significant differences in crop phenological stages, growth, and yield parameters across years and locations for tested traits. Additive Main Effect and Multiplicative Interaction(AMMI) and Genotype and Genotype × Environment (GGE) biplot analysis were used to study genotype by environmental interaction(GEI). Genotype, location, and year interactions significantly influenced all durum wheat genotypes. The combined analysis of variance for all tested traits including grain yield, showed highly significant effects (p < 0.001). Grain yield ranged from 4.5 t ha-1in Dabat to 2.29 t ha-1in Simada. Among the genotypes, Tesfaye had the highest mean yield (3.74 t ha-1), while Wehabit had the lowest (2.75 t ha-1). The AMMI analysis revealed that most of the variation was due to environment (45%), followed by genotype (16%) and genotype × environment interaction (16%), showing strong environmental influence on yield performance. The GGE biplot and AMMI analysis identified Tesfaye and Don metteo as stable genotypes, while Simada and Bichena as representative environments. This study identifies Tesfaye as the most productive and stable wheat variety in the study area and suggests farmers should adopt it to increase wheat yield per unit area of land.

## Full-text entities

- **Chemicals:** PONE-D-25-30070R2 (-), -D (MESH:D003903), NPS (MESH:D009405), urea (MESH:D014508), phosphorus (MESH:D010758)
- **Species:** Triticum aestivum (bread wheat, species) [taxon 4565], Oryza sativa (Asian cultivated rice, species) [taxon 4530], Homo sapiens (human, species) [taxon 9606], Triticum turgidum (cone wheat, species) [taxon 4571], Triticum turgidum subsp. durum (durum wheat, subspecies) [taxon 4567], Sorghum bicolor (broomcorn, species) [taxon 4558]

## Full text

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## Figures

6 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12987450/full.md

## References

88 references — full list in the complete paper: https://tomesphere.com/paper/PMC12987450/full.md

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Source: https://tomesphere.com/paper/PMC12987450