# Effects of protein sources at sahur on anaerobic power and strength during Ramadan in combat sport athletes: A single blind, randomized, placebo-controlled, counterbalanced crossover study design

**Authors:** Abdullah Demirli, Süleyman Ulupınar, Ayşe Türksoy Işım, Alay Kesler, Merve Terzi, Cebrail Gençoğlu, Serhat Özbay, Melenco Ionel, Ibrahim Ouergui, Luca Paolo Ardigò, Leonardo Andreato, Fenghua Sun, Fenghua Sun

PMC · DOI: 10.1371/journal.pone.0340961 · PLOS One · 2026-03-13

## TL;DR

This study examines how different protein sources at sahur affect anaerobic power and strength in combat sport athletes during Ramadan fasting.

## Contribution

The study introduces a novel crossover design to compare the effects of whey and casein proteins on athletic performance during Ramadan.

## Key findings

- Ramadan fasting significantly reduced anaerobic power and strength compared to non-fasting conditions.
- Micellar casein supplementation partially mitigated fasting-induced performance declines better than whey protein and placebo.
- Protein timing and type at sahur can influence athletic performance during fasting.

## Abstract

This study investigated the acute effects of different protein sources consumed at sahur on anaerobic power and strength performances in trained male combat sport athletes during Ramadan fasting. Using a single blind, randomized, placebo-controlled, counterbalanced crossover study design, 24 male combat sports’ athletes (mean age: 27.3 ± 3.8 years, Tier 3 national level) completed four experimental conditions: (1) non-fasting control, (2) fasting + placebo (maltodextrin), (3) fasting + whey protein isolate (WPI), and (4) fasting + micellar casein (MC). In each condition, a standardized sahur meal (6.3–7.7 kcal/kg body weight) and supplementation (0.4 g/kg for WPI/MC and 0.4 g/kg for Placebo) were administered. Physical Performances was assessed 11–13 hours post-sahur (or 3–5 hours post-lunch for control) including the Wingate anaerobic test, bench press, leg press, and countermovement jump (CMJ), and handgrip strength tests. Ramadan fasting significantly lowered Wingate peak power, mean power, and bench press strength compared to the non-fasting control. MC supplementation reduced these declines, outperforming WPI and the placebo in peak power and mean power, and surpassing the placebo in bench press strength, although not WPI. Leg press, countermovement jump, and handgrip strength showed no significant differences across conditions. MC supplementation at sahur provides partial protection against fasting-induced declines in anaerobic power and upper body endurance, but does not fully restore performance to non-fasting levels. These findings emphasize the importance of protein timing and selection in mitigating performance decrements during Ramadan fasting, highlighting the need for further research on optimal nutritional strategies for athletes training and competing under fasting conditions.

## Full-text entities

- **Genes:** MTOR (mechanistic target of rapamycin kinase) [NCBI Gene 2475] {aka FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS}, SLC17A5 (solute carrier family 17 member 5) [NCBI Gene 26503] {aka AST, ISSD, NSD, SD, SIALIN, SIASD}
- **Diseases:** fatigue (MESH:D005221), dehydration (MESH:D003681), loss (MESH:D016388), CMJ (MESH:C000711648)
- **Chemicals:** ATP (MESH:D000255), carbohydrate (MESH:D002241), water (MESH:D014867), aluminum (MESH:D000535), caffeine (MESH:D002110), lactose (MESH:D007785), amino acid (MESH:D000596), glycogen (MESH:D006003), leucine (MESH:D007930), Anti (-), maltodextrin (MESH:C008315)
- **Species:** Homo sapiens (human, species) [taxon 9606], Bos taurus (bovine, species) [taxon 9913], Citrus x limon (lemon, species) [taxon 2708]

## Full text

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## Figures

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## References

46 references — full list in the complete paper: https://tomesphere.com/paper/PMC12987419/full.md

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Source: https://tomesphere.com/paper/PMC12987419