# Methylome and Transcriptome Analysis Reveals Differences in Callus Development and Plantlet Regeneration Capacity Between Two Eucalyptus Species

## TL;DR

This study compares two Eucalyptus species to understand differences in their ability to regenerate plantlets through callus development using methylome and transcriptome data.

## Contribution

The study identifies key genes and pathways involved in callus development and regeneration capacity differences between two Eucalyptus species.

## Key findings

- Differentially expressed genes were enriched in plant hormone signal transduction and MAPK signaling pathways.
- Genes like AHP, BAK1, and CRE1 are potentially involved in callus development and regeneration.
- Observed differences provide a molecular basis for studying Eucalyptus regeneration mechanisms.

## Abstract

Eucalyptus is a highly diverse genus of the Myrtaceae family that is planted worldwide. Many changes occur during callus development, an important process during in vitro plant regeneration. In this study, we conducted methylome and transcriptome analyses to reveal such changes. The results showed that differentially expressed genes between E. camaldulensis (voucher ID: c0009; high embryogenic potential) and E. grandis × urophylla (voucher ID: j0017; low embryogenic potential) during callus development were enriched in plant hormone signal transduction and MAPK (Mitogen-activated protein kinase) signaling pathways. qRT-PCR analysis showed AHP, BAK1, BSK, CRE1, GID1, MKS1, PR-1, PYL, RbohD, and TCH4 could be involved in the callus development and plantlet regeneration capacity. The differences observed in regenerative potential during callus maturation between the two species under study provide a reliable molecular basis for the study of Eucalyptus regeneration mechanisms.