# A Fluorescent Aptasensor Based on Tb-MOFs and Its Application for the Detection of Pseudomonas aeruginosa in Foods

## TL;DR

This paper introduces a new fluorescent sensor for detecting Pseudomonas aeruginosa in food and drinks, offering a fast and sensitive method to ensure food safety.

## Contribution

The novel contribution is a Tb-MOFs-based fluorescent aptasensor for rapid and sensitive detection of Pseudomonas aeruginosa.

## Key findings

- The sensor shows a linear detection range from 1 to 106 CFU/mL of Pseudomonas aeruginosa.
- The detection limit is as low as 0.63 CFU/mL.
- The sensor was successfully applied to bottled water and orange juice samples.

## Abstract

Pseudomonas aeruginosa is an important waterborne pathogen that is harmful to food safety and human health. Therefore, the detection of Pseudomonas aeruginosa has become an essential item in food sampling. This article constructs a fluorescent aptamer sensor based on Tb-MOFs, using Tb-MOFs as the fluorescence signal source, colloidal gold (AuNPs) as the signal conversion switch, and aptamers as the target recognition element, to establish a quantitative detection method for Pseudomonas aeruginosa. The constructed sensor exhibits a good linear relationship within the concentration range of Pseudomonas aeruginosa from 1 to 106 CFU/mL, with a detection limit of 0.63 CFU/mL. Moreover, the sensor was also applied to the detection of Pseudomonas aeruginosa in actual samples of bottled drinking water and orange juice. The fluorescent aptasensor based on Tb-MOFs provides a rapid and sensitive new sensing method for the detection of Pseudomonas aeruginosa in food.