# Process Optimization of GABA Instant White Tea Based on Response Surface Methodology and Analysis of Key Flavor Substances

**Authors:** Dihan Yang, Teng Wang, Wenwen Jiao, Qiuyue Chen, Nianguo Bo, Yiqing Guan, Bin Jiang, Hongyan Gao, Xiaying Tao, Fan Yang, Ping Liang, Bei Cai, Guanghong Pan, Yingling Zhou, Chunyan Zhao, Ming Zhao

PMC · DOI: 10.3390/foods15050967 · Foods · 2026-03-09

## TL;DR

This study improves the production of GABA instant white tea by optimizing processing parameters, enhancing both its nutritional content and flavor profile.

## Contribution

The novel integration of response surface methodology with spray-drying optimization enhances functional components and sensory quality in GABA instant tea.

## Key findings

- Optimized parameters increased GABA content by 209% and enriched theanine, catechins, and caffeine by 200–300%.
- Volatile profile analysis identified epoxy-β-ionone and linalool as key contributors to the tea's aroma.
- The process enables large-scale production of functional tea with improved quality characteristics.

## Abstract

This study developed an optimized processing strategy for γ-aminobutyric acid (GABA) instant white tea (GABA-IT) using GABA-enriched white tea as raw material, systematically characterizing its chemical composition and volatile profile. In contrast to the conventional instant tea production process, this work integrates response surface methodology with spray-drying parameter optimization. This integrated approach enables the simultaneous enhancement of functional components and sensory quality. A response surface design was employed to refine the extraction and spray-drying variables following preliminary single-factor trials, and the optimal parameter combination was subsequently determined (40% ethanol concentration, material-to-liquid ratio of 1:15, extraction time of 3 days, atomization speed of 300 rpm, and inlet temperature of 120 °C); the resulting GABA-IT exhibited significantly improved quality characteristics. Specifically, the GABA content increased by 209% (reaching 4.42 mg/g), and theanine, catechins, and caffeine were enriched by 200–300%. Regarding volatile profiles, processing led to a reduction in esters but an increase in aldehydes and hydrocarbons. Relative odor activity value (rOAV) analysis revealed that epoxy-β-ionone and linalool were the key contributors to the characteristic aroma of GABA-IT. Collectively, this study demonstrates the technical feasibility of producing GABA-rich instant tea with enhanced functional components and improved sensory quality, providing practical guidance for the large-scale industrial production of functional tea beverages.

## Linked entities

- **Chemicals:** γ-aminobutyric acid (PubChem CID 119), GABA (PubChem CID 119), theanine (PubChem CID 439378), catechins (PubChem CID 1203), caffeine (PubChem CID 2519), linalool (PubChem CID 6549)

## Full-text entities

- **Chemicals:** hydrocarbons (MESH:D006838), theanine (MESH:C026166), catechins (MESH:D002392), ethanol (MESH:D000431), caffeine (MESH:D002110), linalool (MESH:C018584), esters (MESH:D004952), aldehydes (MESH:D000447), GABA Instant White Tea (-), GABA (MESH:D005680)

## Full text

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## Figures

5 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12985014/full.md

## References

34 references — full list in the complete paper: https://tomesphere.com/paper/PMC12985014/full.md

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Source: https://tomesphere.com/paper/PMC12985014