# Characteristics of Translocation, Distribution, and Transformation of the Nematicide Fluopyram in Cucumber and Tomato Seedlings and Risk Assessment Based on QSAR Model Prediction

## TL;DR

This study examines how the nematicide fluopyram is absorbed, distributed, and transformed in cucumber and tomato plants, and assesses its environmental risks.

## Contribution

The study identifies fluopyram's metabolic pathways and evaluates the toxicity of its metabolites using QSAR model predictions.

## Key findings

- Fluopyram is highly absorbed by roots and extensively transformed in cucumber and tomato plants.
- 16 metabolites were identified, with most accumulating in leaves and potentially edible parts.
- QSAR predictions show fluopyram and some metabolites pose risks to aquatic ecosystems.

## Abstract

Fluopyram is a widely used nematicide with a growing number of varieties registered both domestically and overseas. However, its absorption, transportation, and metabolism behaviors in plants have not been fully elucidated, thus hindering comprehensive assessment of the risks associated with its use. This study investigated the plant uptake, distribution, and metabolic behavior of fluopyram through 168 h hydroponic experiments. Fluopyram was easily absorbed by the roots of the tested crops, and almost 90.5% and 70.9% of fluopyram was transformed in cucumber and tomato, respectively, leading to the tentative identification of 16 metabolites using Quadrupole Time-of-Flight mass spectrometry. The metabolic reactions involved were hydroxylation, hydroxylation–dechlorination, dehydrogenation, dechlorination, and glucuronidation conjugation. Most metabolites were detected in leaves, suggesting that they have considerable potential to accumulate in the upper parts, even the edible parts. Model prediction indicated that fluopyram and high-toxicity metabolites (M430A, M412C) pose significant risks to aquatic ecosystems across trophic levels, while M574A and M574B showed reduced toxicity due to glucuronidation conjugation. These findings deepen our understanding of the behavioral characteristics of fluopyram within plants, and serve as an important reference for comprehensively assessing its risks.