# CircRNA AIDA Regulates Development of Bovine Myoblast via Binding miR-29a

## TL;DR

A circular RNA called circAIDA helps control muscle development in cattle by acting as a sponge for miR-29a, affecting cell growth and muscle regeneration.

## Contribution

Discovery of a novel circAIDA/miR-29a regulatory axis in bovine skeletal muscle development.

## Key findings

- circAIDA promotes myoblast proliferation and inhibits apoptosis and differentiation in vitro.
- circAIDA attenuates skeletal muscle regeneration in vivo by sponging miR-29a.
- circAIDA relieves miR-29a repression on AKT3 and CLCN2 genes.

## Abstract

What are the main findings?
A novel circAIDA was identified as a miR-29a sponge that relieves the repression of AKT3 and CLCN2.circAIDA promotes myoblast proliferation and inhibits apoptosis and differentiation in vitro, while weakening skeletal muscle regeneration in vivo.

A novel circAIDA was identified as a miR-29a sponge that relieves the repression of AKT3 and CLCN2.

circAIDA promotes myoblast proliferation and inhibits apoptosis and differentiation in vitro, while weakening skeletal muscle regeneration in vivo.

What are the implications of the main findings?
This study reveals a novel circAIDA/miR-29a axis that regulates bovine skeletal muscle development.These findings provide new insights into the molecular mechanisms governing muscle growth and regeneration in bovine.

This study reveals a novel circAIDA/miR-29a axis that regulates bovine skeletal muscle development.

These findings provide new insights into the molecular mechanisms governing muscle growth and regeneration in bovine.

Circular RNAs (circRNAs) have emerged as critical regulators of skeletal muscle development, yet the functions of many muscle-derived circRNAs remain uncharacterized. In this study, we identified a novel circRNA, circAIDA, formed by exons 2 to 6 of the AIDA gene, based on bovine muscle sequencing data. Mechanistically, we demonstrate that circAIDA acts as a molecular sponge for miR-29a, thereby relieving the repression of its downstream targets, AKT3 and CLCN2. Functionally, circAIDA promotes bovine myoblast proliferation while inhibiting apoptosis and differentiation in vitro. Furthermore, experiments in vivo suggested circAIDA could attenuate regeneration of skeletal muscle in mice. In brief, we discovered a novel circAIDA/miR-29a interaction that regulates bovine myogenesis, providing new insights into the molecular networks controlling skeletal muscle development.