Discovery of Exoticoumarins A–L: New Anti-Inflammatory Coumarin Derivatives from Murraya exotica
Feng Wu, Zhan-Tao Zhao, Dai-Lin Tang, Bing-Ying Zheng, Nan An, Long Jiang, Lu Gan, Sheng Yin, Jia-Luo Huang, Gui-Hua Tang
TL;DR
Researchers discovered twelve new anti-inflammatory compounds from Murraya exotica, with two showing strong potential to inhibit inflammation by targeting specific cellular pathways.
Contribution
The discovery of twelve new coumarin derivatives, including exoticoumarins A and K, which potently inhibit inflammation through JNK pathway suppression.
Findings
Exoticoumarins A and K inhibited nitric oxide production with IC50 values of 7.41 and 10.63 μM.
Exoticoumarin A suppressed iNOS expression at both transcriptional and translational levels.
The anti-inflammatory effect of exoticoumarin A was linked to JNK pathway inhibition without affecting ERK 1/2 phosphorylation.
Abstract
The ethanolic extract of the roots of Murraya exotica (Rutaceae) yielded twenty coumarins, including twelve previously undescribed compounds named exoticoumarins A–L (1–12; two biscoumarins, five coumarin hybrids, and five monomers). Their structures, including absolute configurations, were elucidated by a combination of NMR and HR-ESI-MS analyses, single-crystal X-ray diffraction, ECD exciton coupling, Mo2(OAc)4- and Rh2(OCOCF3)4-induced ECD, comparison of experimental with calculated ECD spectra, and chemical hydrolysis. Anti-inflammatory evaluation in LPS-stimulated RAW264.7 macrophages identified exoticoumarins A and K (1 and 11) as potent inhibitors of nitric oxide (NO) production, with IC50 values of 7.41 and 10.63 μM, respectively. Mechanistic studies revealed that 1 suppressed nitric oxide synthase (iNOS) expression at both transcriptional and translational levels, an effect…
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Taxonomy
TopicsPlant chemical constituents analysis · Synthesis and biological activity · Bioactive natural compounds