Preliminary Exploration of Structure-Immunostimulatory Activity Correlation of Spherical Pectin from Chrysanthemum Tea Infusion
An Peng, Mouming Zhao, Lijun You, Lianzhu Lin
TL;DR
This study explores how the structure of spherical pectin from chrysanthemum tea relates to its ability to stimulate the immune system.
Contribution
The study identifies the RG-I domain and highly branched arabinan chains as key structural features linked to immunostimulatory activity in spherical pectin.
Findings
The RG-I domain is crucial for the immunostimulatory activity of spherical pectin.
Highly branched arabinan chains in spherical pectin activate immune cells via TLR4 recognition.
Molecular docking shows that specific arabinose branches bind to TLR4/MD-2 complexes, stabilizing their structure.
Abstract
The spherical pectin is an important bioactive component of chrysanthemum tea infusion, but its biological function, primary structure, and structure-activity relationship remain unclear. The present study evaluated the immunostimulatory activity of spherical pectin from Chrysanthemum morifolium Ramat. ‘Hangbaiju’ tea infusion in RAW264.7 cells and preliminarily investigated its structure-immunostimulatory activity relationship. The rhamnogalacturonan-I (RG-I) domain played a key role in the immunostimulatory activity of spherical pectin. Terminal and branched arabinose residues together accounted for 73.8% of the total arabinose residues in spherical pectin, indicating that the arabinan chains of spherical pectin were highly branched. The backbone of these arabinan chains consisted of →5)-α-Araf-(1→ repeats, and additional →5)-α-Araf-(1→ branches were linked to the backbone via…
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Taxonomy
TopicsPolysaccharides and Plant Cell Walls · Transgenic Plants and Applications · Ginseng Biological Effects and Applications