Implantation of autogenic and decellularized xenogenic grafts for tissue repair in experiment

TL;DR

This study shows that using autogenic and decellularized xenogenic grafts can significantly improve tissue regeneration in severe skin burns in rats.

Contribution

The study introduces a promising grafting method that enhances epithelialization and wound healing in experimental burn models.

Findings

Implantation of grafts led to tenfold more regeneration sites compared to control zones.

Over 90% epithelialization was observed in the experimental zone, versus less than 10% in the control.

Burn wounds healed with minimal scarring when using the grafts.

Abstract

With extensive tissue damage, the body is unable to restore their integrity on its own. Implantation of autogenic and decellularized xenogenic grafts opens up new possibilities for regeneration of damaged corresponding tissues.

The pilot experimental study was conducted on a model of healing of grade III B skin burn wounds in Wistar rats. After removal of the necrotized tissues, autogenous and decellularized xenogenic grafts were implanted into the blood-supplying tissues of the burn wounds.

The pilot experimental study showed that implantation of autogenic and decellularized xenogenic grafts in the experimental zone led to the formation of multiple regeneration sites, almost ten times higher than the marginal epithelialization of the control zone. The proportion of epithelialization of the experimental zone initiated by the installed grafts was more than 90%, and the proportion of marginal epithelialization of the control zone was less than 10%. The completion of epithelialization of skin burn wounds with a predominance of epithelialization of the experimental zone led to the healing of burn wounds. The tightening of the wound edges by scar tissue was minimal.

Implantation of autogenic or decellularized xenogenic grafts can potentially be used to repair any tissues after their damage or disease. The results obtained are preliminary, requiring verification on a wider sample of experimental animals. The use of this methodology to repair tissues with a more complex structure than the skin, for increase the functioning of the parenchyma of various organs requires further study.

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