# Spatial alignment of chemoarchitecture and resting-state functional connectivity predicts short term weight restoration in anorexia nervosa

## TL;DR

This study explores how brain connectivity and neurotransmitter levels relate to weight recovery in anorexia nervosa patients.

## Contribution

The study introduces spatial alignment of chemoarchitecture and functional connectivity as a predictor of weight restoration in anorexia nervosa.

## Key findings

- Higher DC in brain regions with VAChT, DAT, and SERT was observed in acutely underweight anorexia patients.
- Weight restoration was linked to normalization of DC in areas with higher DAT density.
- Individual correlations between neurotransmitter densities and DC predicted early weight gain during treatment.

## Abstract

Resting-state functional connectivity (rsFC) studies have revealed altered regional homogeneity (ReHo) and degree centrality (DC) in individuals with anorexia nervosa (AN) compared to healthy controls (HC), but the underlying mechanisms remain unclear. Here we explored the spatial alignment with neurotransmitter receptor and transporter densities (i.e., “chemoarchitecture”, based on “reference” PET studies) as a potential explanatory factor. We investigated rsFC alterations in acutely underweight patients with AN (n = 87) and age-matched HC (n = 87) cross-sectionally at admission and then again after successful weight-restoration treatment. Global ReHo and DC maps were associated with the spatial distribution of neurotransmitter receptors, transporters and/or metabolic glucose uptake. First, the correlation between rsFC alterations in AN and chemoarchitecture was evaluated at the group/timepoint-level. Second, individual-level correlations of neuroreceptor maps with rsFC alterations were calculated to test for possible associations with early weight restoration. The acute state of AN was characterized by higher DC (but not ReHo) in brain regions with a higher cortical density of vesicular acetylcholine transporter (VAChT), dopamine transporter (DAT) and serotonin transporter (SERT). Conversely, weight restoration was associated with normalization of DC, especially in areas with a higher DAT density. Importantly, individual-level spatial correlations between VAChT, DAT and SERT densities and DC alterations at admission significantly predicted early weight gain over first 90 days of treatment. These results suggest that neurochemical context may underlie functional brain alterations, providing a preliminary step toward identifying biological risk signatures. Replication with individualized PET data will be crucial to validate their potential utility for treatment stratification and personalization.