Candida albicans infection suppresses lipopolysaccharide or Pseudomonas aeruginosa stimulated murine bone marrow derived macrophage (BMDM) responses
Christa P. Baker, Stephanie Laba, Jordan Warner, Karen Shepherd, Heather M. Wilson, J. Simon C. Arthur
TL;DR
Candida albicans infection weakens immune responses in macrophages, especially when co-infected with bacteria like Pseudomonas aeruginosa or LPS.
Contribution
This study reveals the suppressive effect of C. albicans on macrophage responses during co-infection using proteomics.
Findings
C. albicans induces a muted immune response in BMDMs compared to LPS or P. aeruginosa.
C. albicans suppresses IL-6 and IL-12p40 secretion and proteomic changes caused by co-infection.
The findings highlight C. albicans' ability to dampen innate immune responses during co-infection.
Abstract
Candida albicans is an opportunistic human pathogen, that commonly causes localised infection of mucosal surfaces. Candida can also cause systemic infections, which remain difficult to treat and have a high risk of mortality. The immune response to C. albicans infection is complex and can be influenced by the surrounding microenvironment, for example, metabolic stresses or co-infection. Macrophages are a key immune cell in defence against C. albicans infection through phagocytosis of C. albicans and cytokine production to co-ordinate immune responses. Here, we utilise Data Independent Acquisition (DIA) based total proteomics to describe the murine bone marrow derived macrophage (BMDM) response to C. albicans infection as well as in response to co-infection with gram-negative bacterial outer membrane component lipopolysaccharide (LPS) or the gram-negative bacteria Pseudomonas aeruginosa.…
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Taxonomy
TopicsAntifungal resistance and susceptibility · vaccines and immunoinformatics approaches · Gut microbiota and health