Analysis of the TIL gene family in Brassicaceae species and functional study of BrTIL1 in cold tolerance
Zhengnan Xu, Xiaolei Tao, Yanxia Xu, Abbas Muhammad Fahim, Yifan Wang, Hao Sun, Shiyi Li, Yuanyuan Zhang, Lijun Liu, Junyan Wu, Wancang Sun, Li Ma
TL;DR
This study explores the TIL gene family in Brassicaceae plants and shows that the BrTIL1 gene helps plants survive cold stress.
Contribution
The study provides the first systematic analysis of the TIL gene family in multiple Brassicaceae species and identifies BrTIL1's role in cold tolerance.
Findings
BrTIL1 is significantly upregulated under low-temperature stress.
Overexpression of BrTIL1 in Arabidopsis improves cold tolerance and enzyme activities.
BrTIL1 interacts with six proteins, some of which show differential expression under cold stress.
Abstract
Temperature-induced lipocalins (TILs) are a class of thermoregulated lipid-transporting proteins crucial for plant stress responses. However, systematic research on the TIL gene family remains relatively limited. In the present study, we conducted a comparative analysis of the TIL gene family in five Brassicaceae species (Arabidopsis thaliana, Brassica rapa L., Brassica rapa subsp. pekinensis, Brassica juncea L., and Brassica napus L.), identifying a total of 23 TIL genes. Analyses of their gene structures, evolutionary relationships, conserved motifs, and cis-acting elements showed extensive collinearity, close homology, and functional conservation, implying they may possess similar biological functions across different Brassicaceae species. The Brassica rapa TIL1 (BrTIL1) gene was significantly upregulated under low-temperature stress. Functional validation showed that Arabidopsis…
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Taxonomy
TopicsLipid metabolism and biosynthesis · Plant Stress Responses and Tolerance · Plant responses to water stress