Structural and functional characteristics of faecal, vaginal and colostrum microbiota in high-yielding sows

TL;DR

This study compares the microbiomes of high-yielding sows with low-yielding ones to understand how microbial communities in feces, vagina, and colostrum affect reproductive success and piglet health.

Contribution

The study identifies distinct structural and functional characteristics of microbiota in high-yielding sows linked to better reproductive performance and neonatal health.

Findings

High-yielding sows have higher microbial diversity and stability in vaginal and colostrum microbiota compared to low-yielding sows.

Functional analysis shows that high-yielding sows have microbiota enriched in beneficial metabolic pathways and antimicrobial activity.

Microbial profiles in high-yielding sows suggest a more favorable environment for neonatal health and reproductive success.

Abstract

Recent studies have highlighted the critical roles of the microbiota in faeces, vagina and colostrum in reproductive success and piglet development. Understanding the structural and functional dynamics of these microbial communities is essential for optimizing the health and productivity of high-yielding (HY) sows. This study aimed to characterize the structural and functional features of these microbial communities in HY sows (litter size>10 piglets) vs. low-yielding (LY) sows (≤10 piglets) and to explore their associations with sow performance and piglet health. Fifteen Yorkshire sows from each group were selected, and fresh faecal, vaginal mucus and colostrum samples were collected post-parturition. Microbial composition was analysed using 16S rRNA gene amplicon sequencing, and functional potential was predicted via PICRUSt2. Results showed that HY sows exhibited higher alpha diversity in vaginal and colostrum microbiota and greater community stability (higher neutral community model R2 values) compared to LY sows. In faeces, HY sows were enriched with Terrisporobacter and Romboutsia, while depleted in Ruminococcus_torques_group. In the vagina, Campylobacter and Peptoniphilus were increased, whereas Eubacterium_nodatum_group decreased. In colostrum, Lactobacillus, Bifidobacterium and Romboutsia were enriched, with reduced Peptostreptococcus, indicating a more beneficial profile for neonatal health. Functional predictions revealed distinct metabolic profiles: HY faecal microbiota upregulated cysteine/methionine metabolism and porphyrin and chlorophyll metabolism; vaginal microbiota enhanced oxidative phosphorylation and thiamine metabolism; colostrum microbiota showed enrichment in monobactam and novobiocin biosynthesis, which is associated with antimicrobial activity and stress response. These findings demonstrate that HY sows harbour a more stable and functionally advantageous microbiome across multiple biological niches, which may contribute to superior reproductive performance and improved offspring health. The results provide a foundation for developing microbiome-targeted strategies to enhance productivity and welfare in commercial pig production.

Figures