Integrative transcriptomics unravels the central role of fatty acid elongation in early cotton fiber development

TL;DR

This study shows that fatty acid elongation plays a key role in the early development of cotton fibers, offering new insights for improving cotton quality through genetic breeding.

Contribution

The study identifies a novel model linking fatty acid metabolism to cotton fiber initiation and highlights key genes involved in this process.

Findings

A gene module highly correlated with fiber development is enriched for lipid metabolism pathways.

83 hub genes, including key enzymes and transcription factors, were identified in the network.

Very-long-chain fatty acid biosynthesis is essential for cotton fiber initiation and early development.

Abstract

Cotton fiber initiation is a critical determinant of yield and quality. To decipher the genetic networks underlying this process, we conducted a time-series transcriptome analysis of a wild-type cotton (142-WT) and its fuzzless-lintless mutant (142-fl) at 0, 1, and 2 days post-anthesis (DPA). Phenotypic characterization confirmed the absence of fiber protrusion in the mutant. Through integrated analysis of differentially expressed genes (DEGs) and weighted gene co-expression network analysis (WGCNA), we identified a key module highly correlated with fiber development. This module is overwhelmingly enriched for lipid metabolism pathways, notably fatty acid elongation. Furthermore, we identified 83 high-connectivity hub genes within this network, including key enzymes like 3-ketoacyl-CoA synthases (KCS) and regulatory transcription factors. Our findings propose a novel model in which the coordinated activation of fatty acid metabolism, particularly the biosynthesis of very-long-chain fatty acids, is essential for the initiation and early development of cotton fibers. This study provides crucial insights and valuable genetic resources for the molecular breeding of cotton with improved fiber traits.

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