Development and validation of a synthetic peptide-based field deployable latex slide agglutination test for detection of Tilapia tilapinevirus
Meshram Supradhnya Namdeo, Megha Kadam Bedekar, Jeena Kezhedath, Kooloth Valappil Rajendran, Anisha Valsalam, Ankita Godavarikar, Pooja Vinde, Sagar Hundare, Khangembam Victoria Chanu, Dimpal Thakuria, Narrotam P. Sahu
TL;DR
Researchers developed a fast and easy test to detect a virus in tilapia fish, which could help protect the global tilapia industry.
Contribution
A novel synthetic peptide-based latex slide agglutination test for field detection of Tilapia tilapinevirus (TiLV) was developed and validated.
Findings
The TiLV-LAT assay achieved 88.37% specificity and 82.37% sensitivity for TiLV detection.
The assay successfully detected TiLV in various tissues with variable sensitivity.
The test offers a rapid, field-deployable alternative to nucleic acid-based assays.
Abstract
Tilapia significantly contributes to global food security and is an affordable protein source for most developing nations. Tilapia tilapinevirus (TiLV) poses a significant economic threat to the global tilapia industry. This study aimed to develop a rapid and accurate detection method for TiLV by synthesizing a monoclonal antibody (MAb) against it. A novel peptide, KLH-CQ, derived from the TiLV sequence, was designed considering physicochemical properties like net cationic charge, amphipathicity, helicity, and hydrophobicity. The KLH-CQ (50 µg) was used to immunize Balb/c mice with Freund's complete adjuvant. The presence of specific antibodies in the mice serum was confirmed by ELISA, which showed a high antibody titre of 2.67:0.12 (mean OD of treated Vs control sera). The mouse with the strongest immune response was used for spleen donor in hybridoma production. Epitope mapping via…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16
Figure 17
Figure 18Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsVirology and Viral Diseases · Mosquito-borne diseases and control · Animal Disease Management and Epidemiology