# Complete genome sequence of the calcite-precipitating bacterium Cytobacillus kochii strain ITBMC_116, isolated from a limestone karst in Vietnam

**Authors:** Thiet M. Vu, Loan Q. Le, Thanh M. Luc, Khoa H. D. Do, Danh H. Nguyen, Kien T. Tran, Nhung T. T. Vu, Dung H. Nguyen

PMC · DOI: 10.1128/mra.01367-25 · 2026-02-11

## TL;DR

This paper presents the complete genome of a calcite-precipitating bacterium isolated from a limestone karst in Vietnam.

## Contribution

The study provides a complete genome sequence of Cytobacillus kochii strain ITBMC_116 using hybrid sequencing.

## Key findings

- The genome includes a 4.83-Mbp chromosome and an 87.4-kbp plasmid.
- The sequence supports research on microbially induced calcite precipitation and biomineralization technologies.

## Abstract

We report the complete genome of calcite-precipitating Cytobacillus kochii strain ITBMC_116, isolated from limestone karst in Vietnam. Hybrid Illumina–Nanopore sequencing yielded a 4.83-Mbp chromosome and an 87.4-kbp plasmid. This resource supports studies of microbially induced calcite precipitation and biomineralization-based biotechnologies.

## Linked entities

- **Species:** Cytobacillus kochii (taxon 859143)

## Full-text entities

- **Chemicals:** calcite (MESH:D002119)

---
Source: https://tomesphere.com/paper/PMC12981140