# CtGAP 2.0: enhanced genome assembly, strain resolution, and phylogenomics for Chlamydia trachomatis

**Authors:** Olusola Olagoke, Timothy D. Read, Deborah Dean

PMC · DOI: 10.1128/mra.01470-25 · 2026-02-24

## TL;DR

CtGAP 2.0 is an improved tool for analyzing Chlamydia trachomatis genomes, offering better assembly, strain resolution, and phylogenomics.

## Contribution

CtGAP 2.0 introduces a unified installation, automatic quality scoring, and integrated typing and phylogenomics for Chlamydia trachomatis.

## Key findings

- CtGAP 2.0 simplifies the workflow by eliminating the need for multiple standalone tools.
- The tool includes automatic assembly quality scoring to improve downstream analysis accuracy.
- It integrates chromosome and plasmid typing with phylogenomics for comprehensive genomic analysis.

## Abstract

We present Chlamydia trachomatis Genome Assembly Pipeline (CtGAP) 2.0, an enhanced version of CtGAP, that provides a seamless installation process—eliminating the need for multiple standalone tools—and a streamlined command-line interface, introduces automatic assembly quality scoring and selection for downstream analyses, and integrates chromosome and plasmid typing along with phylogenomics.

## Linked entities

- **Species:** Chlamydia trachomatis (taxon 813)

## Full-text entities

- **Species:** Chlamydia trachomatis (species) [taxon 813]

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12981087/full.md

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Source: https://tomesphere.com/paper/PMC12981087