# Experimental study of platelet-rich fibrin affecting the healing of seawater-impregnated wounds in rabbits

## TL;DR

This study shows that platelet-rich fibrin (PRF) improves healing of seawater-exposed wounds in rabbits by reducing inflammation and promoting blood vessel growth.

## Contribution

The novel finding is that PRF accelerates healing in seawater-impregnated wounds through multiple mechanisms including angiogenesis and anti-inflammatory effects.

## Key findings

- PRF-treated wounds showed 92.9% healing by day 14 compared to 53.5% in controls.
- PRF wounds had reduced inflammation and significantly higher CD34+ neovessel density.
- PRF may inhibit bacterial growth and promote fibroblast activity in seawater-exposed wounds.

## Abstract

To investigate the effect of platelet-rich-fibrin (PRF) on the healing of seawater-impregnated wounds in rabbits and to explore the mechanism.

Twenty-four New Zealand White rabbits were used. Two full-thickness skin wound models were created on each rabbit’s back and immersed in seawater. According to a random number table, one wound on each rabbit was assigned to the PRF group (treated with PRF gel) and the contralateral wound served as the control (untreated). Wound healing rate, histomorphology, bacteriology, and neovascularization (via CD34 immunohistochemistry) were assessed on days 1, 4, 7, and 14 post-operation. Statistical analysis was performed using ANOVA with paired samples t-test and Bonferroni correction.

Wounds in the control group exhibited significant erythema, edema, and inflammatory exudate, with a healing rate of only 53.5% ± 3.2% by day 14. In contrast, PRF-treated wounds showed minimal signs of infection, reduced inflammation, and were almost completely healed (92.9% ± 0.9%) by day 14. The wound healing rate was significantly higher in the PRF group at all time points (P < 0.01). Bacteriological analysis identified BacAutologous PRF significantly promotes the healing of seawater-immersed wounds in rabbits. The mechanism is likely multifactorial, involving the promotion of angiogenesis, reduction of inflammation, and potential inhibition of bacterial growth. PRF represents a promising therapeutic option for the management of seawater immersion wounds. d formation of new capillaries and fibroblasts. Immunohistochemistry confirmed a significantly higher density of CD34+ neovessels in the PRF group at days 4, 7, and 14 (P < 0.05).

Autologous PRF significantly promotes the healing of seawater-immersed wounds in rabbits. The mechanism is likely multifactorial, involving the promotion of angiogenesis, reduction of inflammation, and potential inhibition of bacterial growth. PRF represents a promising therapeutic option for the management of seawater immersion wounds.