Single DNA hairpin nanowire based on self-hybridization chain reaction for sensitive ATP detection
Fengyi Lin, Jing Liu, Yuxin Cheng, Min Li, Hong Zhang, Cuisong Zhou, Yong Guo, Dan Xiao, Peng Mi, Jianyuan Dai
TL;DR
Researchers developed a new DNA-based nanowire that can detect ATP in cells with high sensitivity and selectivity.
Contribution
A single DNA hairpin structure enables self-hybridization chain reaction, simplifying design and improving detection performance.
Findings
The single hairpin design improves signal-to-noise ratio by nearly two-fold compared to traditional HCR.
The SHCR nanowire can detect ATP in living cells and ATP released from dead cancer cells after radiotherapy.
The new design shows better selectivity for single-base mismatches.
Abstract
Hybridization chain reaction (HCR), which typically consists of two hairpins for signal amplification, has emerged as a versatile tool in bioanalytical applications. Here, a novel HCR nanowire based on a single DNA hairpin structure is reported. The hairpin stem is rationally engineered with a palindromic sequence, which enables a self-hybridization chain reaction (SHCR) upon the introduction of the initiator DNA strand. Compared to the conventional two hairpin-based HCR nanowire, the single DNA hairpin-based SHCR nanowire achieves nearly a two-fold improvement in the signal-to-noise ratio and exhibits better selectivity for single-base mismatch. By integrating the initiator DNA strand with adenosine triphosphate (ATP) aptamer, the single DNA hairpin-based nanowire has been applied for sensitive ATP detection, capable of monitoring ATP both in living cells and that released from dead…
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Taxonomy
TopicsAdvanced biosensing and bioanalysis techniques · DNA and Nucleic Acid Chemistry · Nanopore and Nanochannel Transport Studies