Injectable in Situ Cross-linked Oxidized Alginate-Gelatin-Based Hydrogels for Cartilage Tissue Engineering
Lisa Schöbel, Juri Artes, Markus Lorke, Aldo R. Boccaccini
TL;DR
This paper introduces an injectable hydrogel system for cartilage tissue engineering with tunable properties and good cell compatibility.
Contribution
A novel injectable hydrogel system combining covalent and noncovalent cross-linking for cartilage tissue engineering is introduced.
Findings
The hydrogels have tailorable mechanical properties with an effective modulus of 12–20 kPa.
The system demonstrates suitable injectability with injection forces of 3–5 N.
The hydrogels support favorable interactions with chondrogenic ATDC5 cells.
Abstract
The present study introduces an injectable oxidized alginate-gelatin hydrogel system for cartilage tissue engineering, employing a combination of covalent and noncovalent cross-linking mechanisms. Specifically, the network is formed through Schiff’s Base reactions alongside enzymatic and ionic cross-linking. The hydrogels were investigated regarding their mechanical properties, swelling and degradation behavior, injectability, and cytocompatibility. The results indicated tailorable mechanical properties with an effective modulus ranging from 12 to 20 kPa, depending on the enzymatic cross-linker concentration, while demonstrating suitable injectability required for clinical applications with injection forces in the range of 3–5 N. Moreover, the syringe-mixing approach of in situ cross-linked hydrogels showed favorable cell–material interactions with chondrogenic ATDC5 cells.
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsHydrogels: synthesis, properties, applications · Osteoarthritis Treatment and Mechanisms · 3D Printing in Biomedical Research