The VaATG6–VaBI-1 module coordinates ER-associated autophagy and ROS homeostasis for cold tolerance in grapevine
Qiaoling Zheng, Fang Ma, Chang Liu, Qinhan Yu, Ningbo Zhang, Weirong Xu
TL;DR
Researchers found that the VaATG6–VaBI-1 module helps grapevines tolerate cold stress by regulating autophagy and reducing oxidative damage.
Contribution
The study identifies VaATG6 and VaBI-1 as key regulators of cold tolerance through their coordinated role in autophagy and ROS homeostasis in grapevines.
Findings
Cold exposure induces VaATG6 transcription and relocalization, enhancing autophagy and reducing ROS.
Overexpression of VaATG6 improves cold tolerance by boosting antioxidant defenses and sugar accumulation.
Loss-of-function VaATG6 mutants show impaired autophagy and increased cold sensitivity.
Abstract
Cold stress is a major constraint on plant productivity, especially in perennial crops like grapevine (Vitis spp.), where it disrupts cellular homeostasis and induces oxidative damage. Here, we identify VaATG6, a key regulator of autophagy, and its interaction with VaBI-1, an ER-localized modulator, as central components of the cold stress response in Vitis amurensis. Cold exposure rapidly induced VaATG6 transcription and triggered its dynamic relocalization from the nucleus to the ER and autophagosomes, enhancing autophagic flux, reducing reactive oxygen species (ROS) accumulation, and improving cold tolerance. In contrast, VaBI-1 acted as a modulator, fine-tuned autophagy and ROS levels through its interaction with VaATG6. Notably, cold stress strengthened the VaATG6–VaBI-1 interaction, linking a coordinated mechanism that integrated autophagy with ER homeostasis. Overexpression of…
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Taxonomy
TopicsAutophagy in Disease and Therapy · Plant Gene Expression Analysis · Plant Stress Responses and Tolerance