# Cyclover-Assisted Liquid-Phase Peptide Synthesis Using T3P® as a Green Coupling Reagent

## TL;DR

This paper introduces Cyclover, a tag that improves green and efficient liquid-phase peptide synthesis using T3P®.

## Contribution

Cyclover enables tunable, sustainable LPPS with T3P® in 2Me-THF, reducing PMI while maintaining yield and purity.

## Key findings

- Cyclover reduces PMI by ∼2.7-fold in LPPS.
- T3P® maintains high purity and isolated yield in peptide synthesis.
- The method offers a greener route for complex peptides.

## Abstract

Liquid-phase peptide
synthesis (LPPS) continues to evolve toward
more sustainable and efficient methodologies. In this study, we introduce
Cyclover as a tag that enables efficient, tunable LPPS in 2Me-THF
using propylphosphonic anhydride (T3P®) as a coupling reagent.
The extraction protocol reduces the PMI ∼2.7-fold while maintaining
high purity and isolated yield, offering a greener and versatile route
to complex peptides