# Recent Advances in Proximity Labeling-Based Subcellular Proteomic Mapping

## TL;DR

This paper reviews recent developments in proximity labeling techniques that help map proteins within cells with high precision.

## Contribution

The paper highlights novel PL strategies like optoPL and immunoPL that offer spatiotemporal control and non-genetic subcellular profiling.

## Key findings

- Proximity labeling methods now capture multidimensional protein features with subcellular precision.
- Photoactivatable and antibody-targeted PL techniques enable detailed proteome mapping without genetic manipulation.
- Integration with complementary techniques enhances understanding of protein interactions and trafficking.

## Abstract

The spatial organization of the cellular proteome is vital for cellular physiology, as protein localization is closely linked to post-translational modifications, subcellular trafficking, and protein-protein interactions. Systematic profiling of these spatial features can greatly enhance our understanding of protein functions. Recent advances in enzyme-mediated proximity labeling (PL) techniques, such as TurboID and APEX2, have improved our ability to map subcellular proteomes in living cells. This review discusses emerging trends in PL methods, which now offer subcellular precision with multi-dimensional protein features, including post-translational modifications, trafficking, turnover, and interaction with other biomolecules. Additionally, new techniques such as photoactivatable PL (optoPL) and antibody-targeted PL (immunoPL) provide enhanced spatiotemporal control and allow for detailed subcellular proteome mapping without genetic manipulation.

•Functional proximity labeling (PL) enables profiling protein features with subcellular precision.•PL provides valuable insights into protein interactomes with diverse biomolecules.•Photocatalytic PL allows enhanced spatiotemporal mapping of subcellular proteomes.•Antibody-targeted PL facilitates subcellular proteome profiling without genetic manipulation.

Functional proximity labeling (PL) enables profiling protein features with subcellular precision.

PL provides valuable insights into protein interactomes with diverse biomolecules.

Photocatalytic PL allows enhanced spatiotemporal mapping of subcellular proteomes.

Antibody-targeted PL facilitates subcellular proteome profiling without genetic manipulation.

This review highlights recent advances in proximity labeling (PL) technologies, including their integration with complementary techniques to probe multidimensional protein features at subcellular resolution as well as the development of photoactivatable and antibody-targeted PL strategies.