LncRNA VCAN‐AS1 Sponges miR‐374c‐3p to Promote Proliferation, Invasion, Migration, and EMT in Thyroid Cancer
Yan Zhang, Lu Sun, Yuqi Wu, Yingzhao Liu, Hui Jiang, Xiaoluo Chen, Li Wang, Chenguang Wu
TL;DR
This study shows that the long noncoding RNA VCAN-AS1 promotes thyroid cancer growth and spread by interacting with miR-374c-3p.
Contribution
The novel finding is that VCAN-AS1 functions as a competing endogenous RNA by sponging miR-374c-3p to drive thyroid cancer progression.
Findings
VCAN-AS1 overexpression increases thyroid cancer cell proliferation, migration, and invasion.
VCAN-AS1 competitively binds miR-374c-3p, reversing its tumor-suppressive effects.
In vivo experiments confirm the oncogenic role of VCAN-AS1 in thyroid cancer.
Abstract
Thyroid cancer (TC) is the most common endocrine malignancy worldwide, with a rising incidence in recent years. This study investigates the role of long noncoding RNA VCAN‐AS1 (lncRNA VCAN‐AS1) in TC cells and its underlying mechanism. LncRNA sequencing and RT‐qPCR revealed that VCAN‐AS1 expression in clinical TC tissues was significantly higher than in paracancerous tissues. Methods such as CCK‐8, cell scratching, Transwell, and Western Blot were employed to assess the impact of VCAN‐AS1 on TC cells. Overexpression of VCAN‐AS1 promoted TC cell proliferation, migration, and invasion; downregulated the epithelial‐mesenchymal transition (EMT)‐related protein E‐cadherin; and upregulated N‐cadherin, vimentin, slug, and snail. Conversely, silencing VCAN‐AS1 reversed these effects. Sequencing identified differentially expressed miR‐374c‐3p in TC cells between the control and VCAN‐AS1…
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Taxonomy
TopicsCancer-related molecular mechanisms research · Circular RNAs in diseases · Thyroid Cancer Diagnosis and Treatment