# Maintenance of physicochemical, optical, and biological properties of conventional glass ionomer cement enriched with an anacardic acid-derivative compound

## TL;DR

This study shows that adding a compound from cashew nutshells to a dental cement maintains its properties and adds antimicrobial benefits.

## Contribution

The novel use of anacardic acid derivative in glass ionomer cement to enhance antimicrobial activity without compromising key properties.

## Key findings

- LDT11 at 0.5% and 1% significantly reduced S. mutans viability compared to control.
- LDT11 incorporation up to 1% maintained setting time, acid-base reaction efficiency, and surface roughness.
- At 2%, LDT11 increased water sorption and caused visible color changes.

## Abstract

To evaluate the physicochemical, optical, and antimicrobial properties of a conventional glass ionomer cement (GIC) modified with anacardic acid (LDT11).

LDT11, extracted from cashew nutshells, was incorporated into GIC (FX ULTRA, Shofu, USA) at 0.5%, 1%, and 2% (w/w), with 0% as control. Disc-shaped specimens (6–15 mm diameter × 1 mm thickness) were prepared for all evaluations. Setting time (ISO 9917; n = 3), acid-base reaction efficiency (FTIR, COO−/COOH ratio; n = 3), water sorption and solubility (ISO 4049; n = 5), diffusion coefficient (n = 5), surface roughness (Ra, Rz, Rv; n = 5), and color parameters (CIELab, CIEDE2000; n = 5) were measured. Antimicrobial characterizations were carried out, with discs inoculated with Streptococcus mutans UA159 and incubated anaerobically for 7 days (early biofilms) and 14 days (mature biofilms). Biofilms were dyed with the Live/Dead biofilm viability kit and then imaged using confocal laser scanning microscopy (n = 6). Data were analyzed using one-way ANOVA with Tukey post hoc or Kruskal-Wallis with Dwass-Steel-Critchlow-Fligner tests (p < 0.05).

LDT11 incorporation did not significantly affect setting time, acid-base reaction efficiency, solubility, diffusion coefficient, and surface roughness. FTIR spectra revealed no alterations in setting-related functional groups, while LDT11 was identified in the 1000–1100 cm⁻¹ range. At 2%, LDT11 significantly increased water sorption and caused visible color changes (p < 0.05). The 0.5% and 1% groups significantly reduced S. mutans viability compared to control, with the 1% group exhibiting the most pronounced and sustained effect (p < 0.0001).

Incorporation of the anacardic acid derivative up to 1% maintained physicochemical properties of GIC while providing antimicrobial activity.