The complete mitogenome of Tylomelania sp. ‘Poso A’ (Caenogastropoda: Pachychilidae) from Lake Poso assembled from long-read sequencing data

TL;DR

Researchers sequenced the complete mitochondrial genome of a freshwater snail from Lake Poso using long-read sequencing, providing insights into its evolutionary relationships.

Contribution

This study presents the first complete mitogenome of Tylomelania sp. ‘Poso A’ assembled from long-read sequencing data.

Findings

The mitogenome of Tylomelania sp. ‘Poso A’ is 16,540 bp long and closely related to T. sarasinorum from Lake Towuti.

The species is phylogenetically close to T. carbo based on 13 protein-coding and 2 rRNA genes.

This is the second complete mitogenome for the diverse Tylomelania genus and a step toward reference genomes for phylogenomic studies.

Abstract

Sulawesi’s ancient lakes represent biodiversity hotspots for various freshwater groups such as gastropods, shrimps, and crabs, as well as fishes. The less known Lake Poso in the northern part of the island is home to about 25 species belonging to the island-endemic freshwater gastropod genus Tylomelania within the family Pachychilidae. Here, we present the first complete mitogenome of a Tylomelania species from Lake Poso (Tylomelania sp. ‘Poso A’) assembled from long-read (PacBio) sequencing data.

The mitogenome is 16,540 bp long and thus only minimally shorter than the mitogenome of its congener from Lake Towuti, T. sarasinorum (16,632 bp). Based on 13 protein-coding and 2 rRNA genes we place the species within a recently published mtDNA-based phylogeny and show its close relationship to T. carbo.

This is the first Tylomelania mitogenome to be generated using long-read sequencing data, and it is only the second complete mitogenome available for this species-rich radiation. Generating mitogenomes from such long-read data is only the first step towards creating the reference genome required for in-depth phylogenomic analyses.

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