# Development and psychometric validation of the insect fear questionnaire for school-aged children in Iran

**Authors:** Arman Latifi, Mostafa Farahbakhsh, Saideh Yousefi, Somayeh Azimi, Mahasti Alizadeh, Tohid Jafari-Koshki, Aboozar Soltani, Sara Rahimi, Madineh Abbasi

PMC · DOI: 10.1371/journal.pone.0344126 · 2026-03-06

## TL;DR

This study created and validated a questionnaire to measure insect fear and related behaviors in Iranian schoolchildren, showing it is reliable and useful for identifying problematic fears.

## Contribution

A novel, validated questionnaire for assessing insect fear and behaviors in school-aged children in Iran.

## Key findings

- The questionnaire has four factors: knowledge, entomophobia, behavior, and personal fear/anxiety.
- The instrument showed strong psychometric properties with Cronbach’s α of 0.82 and ICC of 0.81.
- The tool is suitable for identifying children with high insect fear or unsafe pesticide practices.

## Abstract

Insect-related fear (Entomophobia) is prevalent in children and affects their behavior and well-being, avoidance behaviors, and potentially hazardous practices such as inappropriate pesticide use. While many tools exist for measuring fear in adults, there is a lack of validated instruments for school-aged children. This study aimed to develop and validate a questionnaire assessing knowledge, entomophobia, behavior, and anxiety about insects among school-aged children in East Azerbaijan, Iran.

This psychometric study involved a multi-stage stratified random sampling design in East Azerbaijan Province, Iran. A researcher-made questionnaire, developed through expert consultation and literature review, was used. A total of 1,370 children participated. Content validity was quantified via CVR and CVI. Construct validity was assessed with exploratory factor analysis (EFA) and confirmatory factor analysis (CFA); EFA and reliability analyses (Cronbach’s alpha, item-total statistics) were performed in IBM SPSS Statistics 25, and CFA was conducted in AMOS 22. Temporal stability was evaluated by test–retest Intraclass Correlation Coefficients (ICC) over a two-week interval (n = 103).

The finalized instrument comprises 32 items loading on four factors: knowledge, entomophobia, behavior, and personal fear/anxiety. EFA and CFA supported the four-factor model with excellent fit (CFI = 0.96, TLI = 0.95, RMSEA = 0.047, SRMR = 0.041). Internal consistency was acceptable (Cronbach’s α = 0.82), and test–retest reliability indicated good temporal stability (ICC = 0.81; 95% CI: 0.76–0.86). No meaningful floor or ceiling effects were observed.

The questionnaire demonstrates sound psychometric properties and is suitable for use in school settings to identify children with elevated insect-related fear or unsafe pesticide practices. Future work should examine criterion validity, measurement invariance across subgroups, and applicability in other cultural contexts.

## Full-text entities

- **Diseases:** Specific phobias (MESH:C562465), anxiety disorders (MESH:D001008), spider (MESH:D013684), Arachnophobia (MESH:C000719193), fear (MESH:C000719212), Anxiety (MESH:D001007), Phobia (MESH:D010698), allergic (MESH:D004342), developmental disorder (MESH:D002658), Entomophobia (MESH:C000719201), spider bites (MESH:D001098)
- **Species:** Araneae (spiders, order) [taxon 6893], Homo sapiens (human, species) [taxon 9606]

## Figures

9 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12965557/full.md

---
Source: https://tomesphere.com/paper/PMC12965557