Compact Spectral Encoding Microscopy by Terrace Grating Optics
Ori Refael Cohen, Reut Orange Kedem, Leonid Leites, Amit Parizat, Jonathan Jeffet, Lior Laufer, Shay Stern, Yuval Ebenstein, Yoav Shechtman
TL;DR
This paper introduces a new type of 3D-printed optical component called Terrace gratings that enable continuous spectral encoding in microscopes without complex setups.
Contribution
The novel Terrace grating optics provide continuous spectral encoding as a plug-and-play add-on for standard microscopes.
Findings
Terrace gratings preserve illumination path and integrate easily with other phase masks in widefield microscopy.
Dual-order Terrace gratings enable robust color decoding with a wavelength-independent reference spot.
The design allows snapshot decoding of multiple colors and simultaneous depth and color readout via PSF shape.
Abstract
Spectral information is essential in microscopy, yet many multispectral imaging solutions require increased optical complexity or restrict the spectrum to a few discrete bands. In this work we introduce the Terrace grating optics family: flat, 3D-printable elements that provide continuous spectral encoding, as a plug-and-play add-on component to standard microscopes. The Terrace design preserves the illumination path in widefield operation and integrates trivially with other microscopy phase masks. We introduce two variants: a Single-order Terrace that concentrates energy into one diffraction order for high signal-to-noise ratio, and a Dual-order Terrace that adds a wavelength-independent reference spot, enabling robust color decoding. Using a Fourier-optics model, we show that the wavelength displacement is linearly controlled by the Terrace step-width and refractive-index mismatch. We…
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Taxonomy
TopicsNear-Field Optical Microscopy · Optical Coatings and Gratings · Advanced Fluorescence Microscopy Techniques