A Novel Compact Multi-Reflecting Time-of-Flight Mass Spectrometer
Anatoly N. Verenchikov, Jason Wildgoose, Sergey N. Kirillov, Aleksey V. Vorobyev, Vasily V. Makarov, Lee A. Gethings, Robert P. Tonge, Matthew E. Daly, William J. Johnson, James I. Langridge
TL;DR
A new mass spectrometer design improves speed, accuracy, and range for analyzing complex biological samples like human blood plasma.
Contribution
A compact multireflecting time-of-flight mass spectrometer with enhanced performance for high-resolution and high-speed analysis.
Findings
The instrument achieves 100,000 FWHM resolution and 100 spectra/s acquisition speed.
It maintains excellent linearity with a dynamic range of 105 and R² = 0.984.
Performance is validated through isotopically labeled lipid analysis in human blood plasma.
Abstract
Mass spectrometry is an indispensable tool for the rapid and in-depth analysis of complex mixtures across diverse biologically important fields including metabolomics, lipidomics, and proteomics. These applications demand high speed instruments with subppm mass measurement accuracy over a wide dynamic range of sample concentrations. Here, we introduce an liquid chromatography–mass spectrometry/MS (LC-MS/MS) quadrupole time-of-flight mass spectrometer featuring a novel collision cell, a high dynamic range detector, and a compact multireflecting orthogonal time-of-flight analyzer. This innovative instrument achieves high analytical performance, acquiring full mass range spectra at 100,000 Full Width Half Maximum (FWHM) resolution up to 100 spectra/s acquisition speed. The instrument achieves excellent linearity within a dynamic range of 105, with a correlation coefficient R 2 = 0.984. The…
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Taxonomy
TopicsMass Spectrometry Techniques and Applications · Metabolomics and Mass Spectrometry Studies · Advanced Proteomics Techniques and Applications