# CENPF Promotes Gastric Cancer Proliferation through c-Myc-Mediated GLS1 Upregulation and Glutamine Metabolism

## TL;DR

This study finds that CENPF promotes gastric cancer growth by boosting glutamine metabolism through c-Myc and GLS1, suggesting CENPF as a potential treatment target.

## Contribution

The study identifies a new CENPF/c-Myc/GLS axis regulating glutamine metabolism in gastric cancer.

## Key findings

- CENPF knockdown reduces gastric cancer cell proliferation and induces apoptosis.
- CENPF enhances glutamine metabolism by stabilizing c-Myc and promoting GLS expression.
- GLS or c-Myc overexpression rescues the inhibitory effects of CENPF knockdown.

## Abstract

Gastric cancer (GC) remains highly lethal, with metabolic reprogramming as a key hallmark. This study explores Centromere Protein F (CENPF)’s role in GC pathogenesis, specifically its regulation of glutamine metabolism.

The Cancer Genome Atlas–Stomach Adenocarcinoma (TCGA-STAD), GSE19826, and GSE27342 datasets were analyzed by bioinformatics to identify key candidate genes in GC. The function of CENPF was assessed by flow cytometry, colony formation assays, and Cell Counting Kit-8 (CCK-8). RNA sequencing, metabolic profiling, chromatin immunoprecipitation (ChIP), western blot (WB), and luciferase reporter assay were employed to investigate the fundamental mechanisms.

CENPF was upregulated in GC tumor samples and had a high diagnostic potential. CENPF knockdown declined cell proliferation, caused G2 arrest, and promoted apoptosis in GC cells. RNA sequencing revealed that CENPF was involved in glutamine metabolism. CENPF overexpression enhanced glutamine consumption and glutamate production, while glutamine deficiency reversed CENPF-mediated cell survival. CENPF stabilized cellular myelocytomatosis (c-Myc) by preventing proteasomal degradation, bound to the glutaminase (GLS) promoter, promoting glutamine metabolism. Overexpression of GLS or c-Myc rescued the CENPF knockdown’s inhibitory effect on GC cell growth.

Our findings identify a new CENPF/c-Myc/GLS axis that affects glutamine metabolism and cell survival in GC, implying that CENPF might be a novel target for the treatment of GC.